Other

Nitto Chemical Industry Co., Ltd.

Bio-polymer Research Co., Ltd.

Takara Shuzo Co., Inc.

Chiyoda Chemical Engineering Construction Co., Ltd.

Amano Pharmaceutical Co., Ltd.

Meito Sangyo Co., Ltd.

Nitto Chemical Co., Ltd.

Teruhiko Beppu

Makoto Nishiyama

Sueharu Horinouchi

Toru Nagasawa

Sueharu Horinouch

Hideaki Yamada

Takayasu Tsuchida

Naoto Tonouchi

Minoru Yoshida

Yoshihiro Hashimoto

Kazutoh Takesako

Fujio Yu

Teruya Nakamura

Norio Shimizu

Takahisa Hayashi

Akira Obayashi

Takakazu Endo

Fumihiro Yoshinaga

Takashi Yamashita

Haruo Machida

Hideshi Yanase

Shinjiro Iwasaki

Yoshinao Koide

Yuji Nakanishi

Toshihiko Higashi

Takeshi Uozumi

Susumu Higashi

Yoshiaki Kurono

Osamu Ikehata

Noboru Ando

Yasushi Iwamoto

Seibun Suzuki

Katsuhiko Nishimori

Noboru Yanagida

Yoshiyuki Kawaguchi

Cellulose-producing microorganism transformed with a gene for an enzyme

The present invention relates to a gene derived from Pseudomonas chlororaphis B23 strain which encodes a polypeptide having nitrile hydratase activity being capable of hydrating nitriles to amides. The invention also relates to a recombinant DNA containing the gene, and a transformant transformed with the recombinant DNA. The present invention further relates to a method of producing nitrile hydratase using the transformant and of amides using nitrile hydratase.

Gene encoding a polypeptide having nitrile hydratase activity, a

The present invention has disclosed the amino acid sequence and nucleotide sequence of the .alpha.- and .beta.-subunits of two types of nitrile hydratase derived from Rhodococcus rhodochrous J-1. The DNA fragment encoding nitrile hydratase is inserted into an expression vector and the recombinant vector is used for transformation. The transformant contains multiple copies of the gene and can produce much higher level of nitrile hydratase compared with conventionally used microorganisms.

DNA fragment encoding a polypeptide having nitrile hydratase activity, a

The present invention provides a recombinant plasmid comprising combining a hybrid plasmid vector with the isolated DNA sequences of one or more genes encoding nitrile degrading enzymes which are derived from bacteria belonging to the genus Rhodococcus, said hybrid plasmid vector comprising an isolated DNA sequence which confers on the vector the ability to replicate and amplify in the cells of bacteria belonging to the genus Rhodococcus, and an isolated DNA sequence which confers on the vector the ability to replicate and amplify in the cells of bacteria belonging to Escherichia coli, and an isolated DNA sequence containing a drug resistance gene.

Hybrid plasmid vectors, recombinant plasmids containing genes encoding

Acetic acid bacterium, plasmid derived from said bacterium and shuttle

A mutant fungus strain which produces a protease with low thermostability and low productivity was made from protease producing fungi and the gene coding for mutant enzyme is isolated from the mutant strain. A promoter which can function in yeast is ligated to the gene and inserted into a plasmid replicable in yeast, and the resulting plasmid is introduced into yeast. The yeast is cultured, thereby producing the mutant enzyme. Furthermore, a gene expressing an enzyme with a far lower thermostability is prepared by site-directed mutagenesis, which is introduced in yeast, thereby producing an enzyme with more distinctively reduced thermostability.

Protease with low thermostability derived from Mucor pusillus

A recombinant DNA capable of being replicated in a bacterium of the genus Pseudomonas is disclosed. The DNA contains a wide host range plasmid vector having a gene that codes for lipase. A process for producing lipase by transforming a host bacterium with the recombinant DNA is also described.

Recombinant DNA, bacterium of the genus Pseudomonas containing it, and

A DNA which encodes polypeptide carrying two specific amino acid sequences and having nitrile hydratase activity; a method for producing nitrile hydratase by incubating a transformant made by the transformation with a recombinant DNA prepared by integrating the foregoing DNA into a vector in a medium and harvesting nitrile hydratase accumulated in the medium; and a method for producing amides by incubating the foregoing transformant and then converting nitriles into corresponding amides by the action of the obtained nitrile hydratase or by making a culture solution, isolates, treated cells or their immobilized products act upon nitriles to produce corresponding amides.

DNA encoding polypeptide having nitrile hydratase activity and method

A thermally stable tryptophanase having (1) an optimum temperature for activity at a pH of 8.0 of about 70.degree. C., and (2) such thermal stability that it is not thermally deactivated when maintained at temperatures up to about 65.degree. C. and a pH of 8.0 for 40 minutes. The thermally stable tryptophanase can be produced by cultivating in a tryptophan-containing culture medium a thermally stable tryptophanase-producing bacterium which does not grow alone in said medium but grows there in the presence of Bacillus sp. strain S, and obtaining the resulting thermally stable tryptophanase from the culture broth. The thermally stable tryptophanase-producing microorganism for use in the above process is a novel organism.

Thermally stable tryptophanase, process for producing the same, and

Expression plasmids containing the full cDNA sequence of calf prochymosin and capable of expressing prochymosin gene in E. coli host cells are disclosed. A method is described for the preparation of said plasmids which comprises altering the spacing between the SD and ATG sequences within the E. coli trp promoter-operator region of the parent expression plasmid, pCR 701, which is known to express prochymosin gene in the E. coli host cells. These modified expression plasmids are designed to provide high expression levels of prochymosin.

Novel expression plasmids containing the full cDNA sequence of calf

The feature of the present invention is a process for preparing compounds represented by general formula (Ia) described below: ##STR1## wherein X.sub.1, X.sub.2, X.sub.3, R.sub.1, R.sub.2, R.sub.3 and R.sub.7 are as defined in formula (I) and R.sub.10 represents a lower alkyl group, or acid salts thereof, which process is characterized by using the guanidylfungins (II) as raw materials, reacting these guanidylfungins with alcohols (III) in the presence of an acid catalyst, and then hydrolyzing the malonic acid monoester (IV).

Guanidylfungin derivatives and their production

A method for inducing the differentiation of tumor cells in human or animal, which comprises administering an effective amount of trichostatin A and/or trichostatin C to the human or animal.

Use of trichostatin compounds for treating tumor cells

A process for the production of sphingomyelinase comprising cultivating a sphingomyelinase-producing microorganism, belonging to the genus Pseudomonas, in a culture medium and recovering sphingomyelinase from the culture medium.

Process for the production of sphingomyelinase

Growing community of inventors

Tokyo, Japan

Teruhiko Beppu