Kaneka Corporation

Ono Pharmaceutical Co., Ltd.

Teruaki Takesue

Yoshihiko Yasohara

Noriyuki Kizaki

Sakayu Shimizu

Michihiko Kataoka

Souichi Morikawa

Takahisa Nakai

Miho Yano

Masaru Mitsuda

Kazumi Okuro

Noriyuki Ito

Susumu Amano

Hiroyuki Kanamaru

Osamu Odawara

Masahiro Funaki

Takeyuki Tsuchidate

Masashiro Funaki

The present invention provides a method for producing a recombinant protein, which can increase the cell growth rate in culture while stably maintaining plasmids in the cells, thereby improving the recombinant protein yield. The present invention provides a method for producing a recombinant protein, including a high-temperature culture step of culturing at 32&#xb0; C. or higher abacterium that contains a gene encoding a recombinant protein, and a low-temperature culture step of culturing thebacterium at a temperature lower than 32&#xb0; C. after the high-temperature culture step.

Method for producing recombinant proteins using recombinant brevibacillus

The present invention relates to a polypeptide having an activity to asymmetrically reduce (3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanone to produce (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol isolated from a microorganism belonging to the genus, a DNA encoding the polypeptide and a transformant that produces the polypeptide. The present invention moreover relates to a method of producing (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol utilizing the polypeptide or the transformant. Using the polypeptide or transformant of the present invention, optically active alcohols such as (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol and the like can be produced efficiently.

Carbonyl reductase, gene thereof and method of using the same

The present invention provides a process for producing an optically active 2-allylcarboxylic acid derivative, which is useful as a pharmaceutical intermediate, from readily available and inexpensive starting materials by the process which can be practiced on a commercial scale in a simple and easy manner, and certain 2-allylcarboxamide derivatives, which are novel and important intermediates in that process. An N-allylcarboxamide derivative undergoes rearrangement reaction diastereoselectively in the presence of a base to give a 2-allylcarboxamide derivative, the resulting derivative is subjected to a carbamation reaction and solvolysis to give an optically active 2-allylcarboxylic acid ester, and then the ester obtained is stereoselectively hydrolyzed using an enzyme to produce 2-allylcarboxylic acid having a high optical purity. In addition, the present invention provides a 2-allylcarboxamide derivative compound which is a novel intermediate in the process of the present invention.

Optically active 2-allylcarboxylic acid derivative and process for producing the same

Growing community of inventors

Takasago, Japan

Teruaki Takesue