Thermo Fisher Scientific Oy

Thermo Finnigan LLC

Other

Thermo Finnegan LLC

Terry N Olney

Vesa Nuotio

John Edward Brann, Iii

Robert Dewitte

Juhani Siidorov

Jukka Saukkonen

Joseph L Herman

Jeffrey A Zonderman

Joseph M Senteno

Bill Ostman

Jarmo Vehkomaki

Raimo Salminen

Harald Oser

Oleg Silivra

Marcel Patek

Alan E Schoen

Joshua T Maze

Martin Smrcina

Frantisek Turecek

Adi A Scheidemann

Peter Strop

Frank J Schumacher, Iv

Daniel Schirlin

John Edward Iii Brann

A method of multiplexing the operation of a sample preparation and analysis system is disclosed. The system includes a sample preparation system capable of preparing a first sample in accordance with a first assay that is selected from a database containing a plurality of unique assays and preparing a second sample in accordance with a second, different assay. The method includes separately transporting the first and second prepared samples to an analysis station having first and second separation channels and an analyzer comprising a mass spectrometer. The method further includes simultaneously analyzing the first prepared sample with the analyzer in accordance with the first selected assay and separating the second prepared sample with the second separation channel in accordance with the second selected assay.

Automated system for sample preparation and analysis

A sample preparation and analysis system. The system includes a sample preparation system and a sample analysis system. The sample preparation system prepares samples in accordance with an assay that is selected from a database containing a plurality of unique assays. The sample analysis system includes an analyzer that is dynamically reconfigurable based on the selected assay so as to analyze the prepared sample in accordance with that selected assay. A data communication link communicates data from the sample preparation system to the sample analysis system to reconfigure the analyzer in accordance with the selected assay.

A sample preparation and analysis system. The systemincludes a sample preparation systemand a sample analysis system. The sample preparation systemprepares samples in accordance with an assay that is selected from a database containing a plurality of unique assays. The sample analysis systemincludes an analyzerthat is dynamically reconfigurable based on the selected assay so as to analyze the prepared sample in accordance with that selected assay. A data communication linkcommunicates data from the sample preparation systemto the sample analysis systemto reconfigure the analyzerin accordance with the selected assay.

A mass spectrometry method comprises: introducing a first portion of a sample of ions including precursor ions comprising a first precursor-ion mass-to-charge (m/z) ratio into a first mass analyzer; transmitting the precursor ions from the first mass analyzer to a reaction or fragmentation cell such that a first population of product ions are continuously accumulated therein over a first accumulation time duration; initiating release of the accumulated first population of product ions from the reaction or fragmentation cell; continuously transmitting the released first population of product ions from the reaction cell to a second mass analyzer; transmitting a portion of the released first population of product ions comprising a first product-ion m/z ratio from the second mass analyzer to a detector; and detecting a varying quantity of the product ions having the first product-ion m/z ratio for a predetermined data-acquisition time period after the initiation of the release.

Method for mass spectrometer with enhanced sensitivity to product ions

A collision cell has a plurality of rod electrodes arranged in opposed pairs around an axial centerline and a plurality of drag vanes arranged in the interstitial spaces between the rod electrodes. Operating the collision cell includes, applying a rod offset voltage to the rod electrodes, and varying an offset voltage applied to the drag vanes to identify a vane offset voltage with a maximum intensity for the transition. The method further includes varying a drag field by adjusting the voltages applied to drag vane terminals in opposite directions to identify a drag field value with a cross talk below a cross talk threshold, varying the vane offset voltage by adjusting the voltages applied to the drag vane terminals to maximize the intensity of the transition while preserving the drag field, and operating the collision cell at the vane offset voltage and drag field to monitor the transition.

Optimizing drag field voltages in a collision cell for multiple reaction monitoring (MRM) tandem mass spectrometry

A sample preparation and analysis system. The system includes a housing with a sample preparation station and a sample analysis station positioned within the housing. The sample analysis station is spaced away from the sample preparation station. A transport assembly is configured to move at least one sample within the housing and between the sample preparation station and the sample analysis station.

System layout for an automated system for sample preparation and analysis

A method for automatically checking and adjusting a calibration of a mass spectrometer having a first quadrupole (Q1), a fragmentation cell and a mass analyzer comprises: introducing a sample having at least one known chemical entity; decreasing a kinetic energy so as to prevent fragmentation of ions in the fragmentation cell; optionally applying a drag field to the fragmentation cell; ionizing the at least one known chemical entity sample to generate a set of ions; performing a mass scan of the set of ions using Q1; transmitting the scanned ions through Q1 to and through the fragmentation cell; detecting the scanned and transmitted ions by a detector of the mass analyzer; and comparing the results with expected results. Embodiments may include automatic recalibration or notification of possible errors, need for further data processing or an analysis of system performance.

Method for automated checking and adjustment of mass spectrometer calibration

A sample preparation and analysis system (). The system () includes a housing () with a sample preparation station () and a sample analysis station () positioned within the housing (). The sample analysis station () is spaced away from the sample preparation station (). A transport assembly () is configured to move at least one sample within the housing () and between the sample preparation station () and the sample analysis station ().

The present invention provides an instrument and methods for the preparative separation of components of mixtures using mass spectrometric methods. Nondestructive ionization methods are employed to generate ionized components of a mixture, the ionized components are spatially separated by mass and the mass-separated ion components are trapped. The ion source and mass spectrometric techniques employed allow the generation of large ion currents of ion components, on the order of nanoamps, which facilitate rapid accumulation of nanomole quantities of mass-separated components in relatively short times (minutes to hours).

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Tracy, CA, United States of America

Terry N Olney