Protein Technologies, Inc.

Argonaut Technologies, Inc.

Sentinel Monitoring Systems, Inc.

Terry D Long

James Richard Wasson

Milan Bier

Anthony R Ford

Christopher Kilcoin

Steve Miller

Glen Ward

Michael J O'Neill

James A Ostrem

Richard Vercillo

Leon E Barstow

Rodolfo Bacheleir Marquez

Steven J Blomquist

Hung Ly

Rodolfo B Marguez

Bill Williams

William H Puent

Marc Zuellig

Gary S Kath

Gregory W King

Brian G Uhrig

Jan Hughes

Steven Hutchins

A flow cytometer can include: at least one light emitter configured to emit light in a light path; a rectangular flow cell having flow cell width that is substantially lateral to the light path and a flow cell depth that is longitudinal to the light path, wherein the light path has an interrogation width at the flow cell that is narrower than the flow cell width; and a spherical reflector positioned adjacent to the rectangular flow cell and having a concave reflective surface that has a reflective direction that is positioned substantially orthogonal with the light path such that reflected light is reflected along a reflected path that is substantially orthogonal with the light path. At least one light absorbing member is positioned at least partially around the reflected path to absorb reflected light at an angle to the reflected path.

Systems and methods for flow cytometry with tailored discrimination

A system may include a needle and source providing fluid to the needle. The system may include a sheath defining an opening. The needle may be within the sheath. The needle and sheath may define a cavity. The system may include a gasket movable between open and closed positions. The gasket may fluidly seal an end of the sheath so that the fluid exits the needle into the cavity and exits via the opening. The system may include a bottle including a septum proximate the end of the sheath. The system may include a movement system that may displace the gasket to permit fluid to exit the cavity via the end of the sheath and displace the sheath or needle such that the needle extends beyond the sheath. The movement system may displace the needle or bottle such that the needle penetrates the septum and fluid exits into the bottle.

Aseptic sampling system

Devices and methods are provides for delivering fluids into reaction vessels. The present device is an interface head which allows a user to add reagents and wash solvents to a reaction vessel. Typically, the interface head can engage a plurality of these reaction vessels mounted in a cassette or frame and is adapted to removably engage passages leading into the reaction vessel. The interface head allows a user to manually inject materials into the plurality of passageways in the head which are fluidly coupled to the reaction vessel. In one embodiment, the interface head has a septa valve which opens and closes inlets of the plurality of passageways. The septa valve comprises an elongate member with a septum portion and a plurality of septum ports. The elongate member is slidable between a first position wherein at least one inlet of the passageways in the interface head is sealed by the septum portion and a second position wherein said at least one inlet is aligned with one of the ports to allow delivery of materials from the inlet into the reaction vessel. The septum portion is penetrable by needles and thus allows access for needle/syringe type delivery devices.

Devices and methods for accessing reaction vessels

Systems and methods for synthesizing chemical compounds using a plurality reaction vessels. In particular, the present invention provides a synthesis apparatus capable of holding a plurality of reaction vessels for parallel synthesis of multiple discrete compounds or for combinatorial libraries of compounds. In one embodiment, a synthesis apparatus comprises a frame having a plurality of reaction vessel-holding openings and a plurality of valves for use in parallel synthesis of a plurality of compounds within reaction vessels.

Systems and methods for parallel synthesis of compounds

The present invention provides apparatus and methods for the synthesis of combinatorial chemical libraries. The apparatus comprises a plurality of reaction vessels having an inner surface, an outer surface, a first opening, and a second opening. An agitator is contained within each of the reaction vessels for stimulating liquid circulation within the vessels. The apparatus has a common gas line having a plurality of gas outlet ports, where each of the reaction vessels has at least one gas outlet port positioned to feed into the vessel. The apparatus also has a common liquid line having a plurality of liquid outlet ports, where each of the reaction vessels has at least one liquid outlet port positioned to feed into the vessel. Each reaction vessel also has at least one valve coupled to the second opening on the vessel.

Apparatus and methods for the preparation of chemical compounds

A multi-channel separation device is provided for use in separation technology, particularly in electrophoresis and in isoelectric focusing. The device includes two mating plates, at least one of which contains an array of at least three cavities arranged to define a serpentine fluid pathway through the cavities when the plates are mated. Each of the cavities includes fluid input and output ports to facilitate transfer of process fluid through the cavities. A septum is disposed between the plates for streamlining fluid flow between the input and output ports by restricting but not preventing free fluid flow between adjacent cavities. The device also includes anodic and cathodic compartments containing electrodes for establishing an electric field across the cavities.

Multi-channel separation device

A novel pH buffering system which is functionally stable under electrophoretic conditions employs buffering components in complementary buffer pairs. The buffering components are selected from among simple chemically defined ampholytes, weak acids and weak bases, and are paired together on the basis of their dissociation characteristics to define narrow overlapping buffering zones in which the buffering components exhibit low net electrophoretic mobility combined with desired pH buffering capacity. The narrow buffering zones cover from 0.4 to 1.25 pH units within a broad range of pH values of from about pH 3 to about pH 10. Specific buffering pH values within the narrow buffering zones are obtainable by varying the molar ratios of the two buffering components of the selected buffer pair. The buffering system may be used to form functionally stable precast narrow pH zone gradients in free solution, and has particular utility in isoelectric focusing and other electrophoretic processes.

Buffering system and its use in electrophoretic processes

A matrix of valves being formed using a sandwich of three layers with a pliable membrane interposed between two of the layers. Supply channels and delivery channels are created through etching, routing, or other such means on the middle layer. Each supply channel communicates with all of the delivery channels via a valve location which is opened or closed by pneumatic pressure on the pliable membrane. Movement of the pliable membrane is affected through suction or pressure as communicated via holes in the overlying layer. In this manner, selected supply and delivery lines are connected by appropriate suction being placed on the membrane. Selected valves are simultaneously opened to provide parallel operations.

Matrix of valves

Growing community of inventors

Tucson, AZ, United States of America

Terry D Long