Topaz Biosciences, Inc.

University of California

Teresa Shock

Oliver Liu

Jennifer Shock

Maritza Miller

James R Craig

Chaoguang Tian

N Louise Glass

Samuel Coradetti

The present disclosure relates to novel cell wall binding domains, enzymatically active domains, and chimeric cell wall hydrolases with anti-activity. The disclosure also relates to compositions comprising these, and uses thereof in the treatment of conditions associated with

Cell wall hydrolases targeting c. acnes

The present disclosure relates to novel chimeric cell wall hydrolases with anti-activity. The disclosure also relates to compositions comprising these chimeric cell wall hydrolases and uses thereof in the treatment of conditions associated withsp.

Recombinant cell wall hydrolases

Provided herein are methods and compositions for increasing the production of one or more cellulases from a fungal host cell. The disclosure is based, on the surprising discovery that mis-expression of the transcriptional regulator clr-2 in a filamentous fungal cell was able to induce expression of cellulase genes under non-inducing or starvation conditions, resulting in increased secretion of cellulases from the cell. Advantageously, mis-expression of the transcription factor clr-2 in a filamentous fungal cell cultured in the absence of cellulose or cellobiose results in increased secretion of cellulases. The disclosure relates inter alia to a method of degrading cellulose-containing material, to a method of increasing the production of one or more cellulases from a fungal cell and to a method of reducing the viscosity of a pretreated biomass material, by contacting pretreated biomass material with a fungal host cell containing at least one recombinant nucleic acid encoding clr-2 or a related transcription factor.

Transcription factors for cellulosic enzyme production

Growing community of inventors

San Francisco, CA, United States of America

Teresa Shock