Henkel Kommanditgesellschaft Auf Aktien

Cognis Corporation

Other

Henkel Konnarditgesellshaft Auf Aktien

Cognis Gesellschaft Fuer Bio Und Umwelttechnologie Mbh

Basf Se Corporation

Teresa M Christianson

Charles R Wilson

Dean W Goddette

Robert B Reynolds

Beth Frances Ladin

Shiow-Shong Yang

Maria R Tang

Karl-Heinz Maurer

Albrecht Weiss

Horst Upadek

Christian G Paech

Irmgard Schmidt

Maria R Lau

Dieter Hansen

Harald Berger

Charles Ronald Wilson

Mutant DSM 5483 proteases are derived by the replacement of at least one amino acid residue of the mature form of the DSM 5483 alkaline protease. The mutant proteases are expressed by genes which are mutated by site-specific mutagenesis. The amino acid sites selected for replacement are identified by means of a computer based method which compares the three dimensional structure of the wild-type protease and a reference protease.

Mutant proteolytic enzymes and method of production

This invention relates to proteases with improved application properties in cleaners and detergents. The improvement's achieved by substituting positively charged or uncharged amino acids in the substrate binding region of the wild type subtilisin protease.

Enzymes for detergents

Mutant B. lentus DSM 5483 proteases are derived by the replacement of at least one amino acid residue of the mature form of the B. lentus DSM 5483 alkaline protease. The mutant proteases are expressed by genes which are mutated by site-specific mutagenesis. The amino acid sites selected for replacement are identified by means of a computer based method which compares the three dimensional structure of the wild-type protease and a reference protease.

This invention discloses an expression system which is useful in industrial Bacilli to produce target proteins which include, but are not limited to, alkaline proteases, amylases, cellulases, lipases or other hydrolyases which are normally excreted outside of the host cell.

Expression systems for the production of target proteins in bacillus

Bacillus lentus alkaline protease varints with enhanced stability

Bacillus lentus alkaline protease variants with increased stability

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Petaluma, CA, United States of America

Teresa M Christianson