National University of Ireland, Galway

Enterprise Ireland

National University of Ireland

Terence James Smith

Thomas Gerard Barry

Justin Joseph O'Grady

Majella Maher

Kate Mary Reddington

Justin O'GRADY

The present invention relates to a multiplex in vitro nucleic acid amplification method for identifying a species of thecomplex present in a sample. The method includes detecting the presence or absence of a plurality of nucleic acid molecule targets in the sample in one reaction, wherein at least one of the nucleic acid molecule targets is present in the genome of one or more, but not all, of the species of thecomplex. The invention also includes kits containing primers or probes for conducting this method, nucleic acids useful for performing this method and diagnostic techniques using these nucleic acids.

Diagnostic method

The present invention use of to a portion of the RPS7 gene or its corresponding mRNA in a diagnostic assay for fungal and yeast species and sequences for use in such assays and methods.

Nucleic acids probes for detection of yeast and fungal

The current invention relates to a diagnostic kit for a bacterial species and/or fungal and/or yeast species comprising at least one oligonucleotide probe capable of binding to at least a portion of the LepA and/or Guf1 genes or its corresponding mRNA.

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Use of the ssrA gene or tmRNA, an RNA transcript of the ssrA gene, or fragments thereof as target regions in a nucleic acid probe assay for the detection and identification of prokaryotic and/or eukaryotic organisms is described. Nucleotide sequence alignment of tmRNA sequences from various organisms can be used to identify regions of homology and non-homology within the sequences which in turn can be used to design both genus specific and species specific oligonucleotide probes. These newly identified regions of homology and non-homology provide the basis of identifying and detecting organisms at the molecular level. Oligonucleotide probes identified in this way can be used to detect tmRNA in samples thereby giving an indication of the viability of non-viral organisms present in various sample types.

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Galway, Ireland

Terence James Smith