Oxford Nanopore Technologies Limited

Terence Alan Reid

James Anthony Clarke

Stuart William Reid

Steven Paul White

Gurdial Singh Sanghera

Lakmal Nishantha Jayasinghe

John Hagan Pryce Bayley

Hagan Bayley

To form a layer separating two volumes of aqueous solution, there is used an apparatus comprising elements defining a chamber, the elements including a body of non-conductive material having formed therein at least one recess opening into the chamber, the recess containing an electrode. A pre-treatment coating of a hydrophobic fluid is applied to the body across the recess. Aqueous solution, having amphiphilic molecules added thereto, is flowed across the body to cover the recess so that aqueous solution is introduced into the recess from the chamber and a layer of the amphiphilic molecules forms across the recess separating a volume of aqueous solution introduced into the recess from the remaining volume of aqueous solution.

Formation of layers of amphiphilic molecules

Nanopore arrays for sequencing nucleic acids

The invention relates to a mutant &#x3b1;-hemolysin (&#x3b1;-HL) pore which is useful for detecting one or more nucleotides by stochastic sensing. The pore is particularly useful for sequencing DNA or RNA. A molecular adaptor that allows detection of the nucleotide(s) is covalently attached to the pore. The pore is specifically modified to facilitate positioning of the adaptor and may be modified to facilitate covalent attachment.

Base-detecting pore

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Kidlington, United Kingdom

Terence Alan Reid