The Hong Kong University of Science and Technology

Shenzhen Nanobioimaging Limited

Teng Zhao

Shengwang Du

Ying Wang

Yumian Su

Ming Tak Michael Loy

Sze Cheung Lau

Michael M T Loy

Methods and systems for generating non-diffracting light sheets for multicolor fluorescence microscopy are disclosed. A method for generating a non-diffracting light patterned Bessel sheet comprises transmitting an input light beam through a Fourier transform lens the input light beam has a spatial intensity pattern at a first plane, and a Fourier plane is formed after the Fourier transform lens to obtain a first light beam; transmitting the first light beam through an annulus mask to obtain a second light beam; and transmitting the second light beam through an excitation objective lens to form a non-diffracting patterned light sheet. A method for generating a non-diffracting light line Bessel sheet comprises transmitting an input light beam at a first lane through an annulus mask to obtain a first light beam; and transmitting the first light beam through an excitation objective lens to form a non-diffracting Bessel light sheet.

Methods and systems for generating non-diffracting light sheets for multicolor fluorescence microscopy

A two-color fluorescence localized super-resolution biological microscopy method and system are disclosed. The method includes: performing two-color fluorescence labeling on a biological sample by using Alexa647 and Alexa750 fluorescent molecules or Cy5 and Cy7 fluorescent molecules, and soaking the biological sample in an imaging buffer solution; illuminating the biological sample by laser to generate a flashing fluorescent signal of a first channel and a flashing fluorescent signal of a second channel respectively; constructing a super-resolution image of a first biological structure and a super-resolution image of a second biological structure respectively; and aligning the super-resolution image of the first biological structure and the super-resolution image of the second biological structure so as to construct a super-resolution image of a third biological structure. Thereby, the present method and system do not generate channel crosstalk and can significantly reduce background noise, so that the imaging quality is improved.

Two-color fluorescence localization super-resolution biological microscopy method and system

Growing community of inventors

Hong Kong, China

Teng Zhao