Lawrence Livermore National Security, LLC

University of California

Ted Alfred Laurence

Shimon Weiss

Joe H Satcher, Jr

Tiziana C Bond

Stephen M Lane

Philip Edward Miller

Salmaan H Baxamusa

Jeffrey Devin Bude

Tayyab Ishaq Suratwala

Michael D Feit

Bradley R Hart

Kathleen Irene Schaffers

William Augustus Steele

John J Adams

Shervin Kiannejad

Thomas R Huser

Matthew A Horsley

Ty James Samo

Lana Louie Wong

Christopher W Hollars

Chad E Talley

Nan Shen

Paul Richard Ehrmann

Nam K Lee

Xiyu Yi

Peter Weber

Achillefs Kapanidis

Marlon G Menor

Paul Ehrmann

John Adams

Michael Dennis Feit

In one inventive concept, a method for etching an optic includes obtaining a microemulsion, where the microemulsion includes a continuous oil phase, a surfactant system comprising at least one surfactant, and water, submerging at least a portion of the optic in the microemulsion, and agitating by ultrasonication the microemulsion for etching the optic submerged therein.

Etching of water-sensitive optics with water-in-oil emulsions

Devices, systems and methods for widefield three-dimensional (3D) microscopy with a quantum entanglement light source are described. An example method includes generating a first set of photons and a second set of photons, wherein each of the photons in the first set is quantum entangled with a corresponding photon in the second set, directing the second set of photons toward a sample and simultaneously directing the first set of photons toward a first two-dimensional (2D) detector, detecting, from the sample, a plurality of photons at a second 2D detector, analyzing detections from the first and second 2D detectors to determine coincidence information, and determining one or more characteristics associated with at least a three-dimensional (3D) section of the sample based on collective detections at the first and the second 2D detectors.

Widefield three-dimensional microscopy with a quantum entanglement light source

A method for preventing damage caused by high intensity light sources to optical components includes annealing the optical component for a predetermined period. Another method includes etching the optical component in an etchant including fluoride and bi-fluoride ions. The method also includes ultrasonically agitating the etching solution during the process followed by rinsing of the optical component in a rinse bath.

Methods for globally treating silica optics to reduce optical damage

A method for analyzing/monitoring the properties of species that are labeled with fluorophores. A detector is used to detect photons emitted from species that are labeled with one or more fluorophores and located in a confocal detection volume. The arrival time of each of the photons is determined. The interval of time between various photon pairs is then determined to provide photon pair intervals. The number of photons that have arrival times within the photon pair intervals is also determined. The photon pair intervals are then used in combination with the corresponding counts of intervening photons to analyze properties and interactions of the molecules including brightness, concentration, coincidence and transit time. The method can be used for analyzing single photon streams and multiple photon streams.

Monitoring molecular interactions using photon arrival-time interval distribution analysis

Fluorescence, spectroscopy is used to analyze small numbers of molecules that are present in a relatively small detection volume or zone. Information regarding physical and chemical properties of these molecules is determined by rapidly modulating the wavelength, intensity and/or polarization of laser energy to excite fluorophores that are attached either to the molecule of interest or a molecule that interacts with the molecule of interest. The emission profile of the fluorophores is used to determine useful information about the labeled and/or non-labeled molecules including molecular interactions between the molecules.

Modulated excitation fluorescence analysis

Surface-Enhanced Raman Spectroscopy (SERS) is a vibrational spectroscopic technique that utilizes metal surfaces to provide enhanced signals of several orders of magnitude. When molecules of interest are attached to designed metal nanoparticles, a SERS signal is attainable with single molecule detection limits. This provides an ultrasensitive means of detecting the presence of molecules. By using selective chemistries, metal nanoparticles can be functionalized to provide a unique signal upon analyte binding. Moreover, by using measurement techniques, such as, ratiometric received SERS spectra, such metal nanoparticles can be used to monitor dynamic processes in addition to static binding events. Accordingly, such nanoparticles can be used as nanosensors for a wide range of chemicals in fluid, gaseous and solid form, environmental sensors for pH, ion concentration, temperature, etc., and biological sensors for proteins, DNA, RNA, etc.

Nanosensors based on functionalized nanoparticles and surface enhanced raman scattering

Growing community of inventors

Livermore, CA, United States of America

Ted Alfred Laurence