Lifesensors, Inc.

Progenra, Inc.

United States of America, as Represented by the Secretary of Agriculture

Tauseef R Butt

Hiep Tran

Oxana A Malakhova

Michael P Malakhov

Steven D Weeks

Stephen R Hughes

Raymond J Peroutka

James Strickler

Xun Zuo

Mabel Alarnino Cejas

William Kingsbury

Amolkumar Karwa

Jeffrey G Marblestone

Tadas Panavas

David E Sterner

Alejandro Bernal

Jian Wu

Míchael P Malakhov

Salam Shaaban

Hossein Askari

Michael Schwartz

Joseph Manimala

Hiep T Tran

Synthetic protease substrates and methods which facilitate the identification of substrates of a protease, particularly ubiquitin, ubiquitin-like, or proteasome protein are provided.

Synthetic protease substrates, assay methods using such substrates and kits for practicing the assay

Methods for enhancing expression levels, secretion, and purification of heterologous fusion proteins in a host cell are disclosed.

Methods and compositions for enhanced protein expression and purification

Recombinantproduced by transformation with heterologous polynucleotide sequences coding for xylulokinase (XKS) fromand xylose isomerase (XI) are capable of xylose utilization. The transformants express these heterologous polynucleotides at a sufficient functional level to grow aerobically on xylose as the sole carbon source. Further transformation of the recombinant yeasts to overexpress one or more of thegenes PIP2, IMG2, MAK5, VPS9, COX10, ALE1, CDC7, and MMS4, permits the yeast to grow anaerobically on xylose as the sole carbon source. When grown under anaerobic conditions on a culture medium comprising both glucose and xylose, the transformed yeast exhibit increased ethanol productivity, with the yeast growing on the xylose to increase their biomass and fermenting the glucose to ethanol.

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The inventive subject matter relates to novel compositions, methods, and kits for enhancing the expression, solubility, and isolation of heterologous proteins. Further, the inventive subject matter relates to methods for generating proteins with novel N-terminal amino acids, unlike wild-type proteins which always are translated from mRNA with methionine at the N-terminus.

Human SUMO-3 for enhancing protein expression

Methods and kits for assessing proteolytic enzyme activity and a modulator's effect thereof employ a Ubiquitin or Ubiquitin-like Protein and a signal producing structure. The corresponding fusion polynucleotide is employed for production of transgenic cells, plants and animals that may be produced by stably transfection, optionally transforming the cell, plant or animal with a Ubiquitin-, UBL- or their C-terminal binding functional fragment-Reporter fusion polynucleotide. A method of diagnosing a disease or condition, comprises contacting or administering a sample obtained from a subject suspected of being afflicted with the disease or condition with the cell, plant or animal, detecting any signal produced by the reporter in the presence of the sample and comparing the signal to controls for 0% and 100% signals.

Method for assessing proteolytic enzyme activity using ubiquitin fusion substrate

Methods for enhancing expression levels and secretion of heterologous fusion proteins in a host cell are disclosed.

Methods and compositions for protein expression and purification

A yeast based transactivation assay for gender sorting is disclosed.

Compositions and methods for gender sorting

A yeast-based system and methods of use therefore are provided for identifying new molecules which activate nuclear receptors in a ligand-dependent fashion. In a preferred embodiment, a method is provided utilizing ecdysone receptor, USP and GRIP I encoding expression vectors which may be used to advantage for screening new and useful insecticidal compounds, detecting insecticidal residues as well as to regulate expression of a gene of interest in a host in a ligand-dependent manner.

Methods and compositions for identifying ligands for nuclear receptors

Growing community of inventors

Malvern, PA, United States of America

Tauseef R Butt