Ajinomoto Co., Ltd.

Tatyana Aleksandrovna Bachina

Shunichi Suzuki

Masakazu Sugiyama

Sergey Vasilievich Smirnov

Tatyana Abramovna Yampolskaya

Veronika Aleksandrovna Kotliarova

Yuri Ivanovich Kozlov

Natalia Nikolaevna Samsonova

Natalia Yurievna Rushkevich

Yuki Imabayashi

Yurgis Antanas Vladovich Iomantas

Elena Georgievna Abalakina

Olga Sergeevna Beznoschenko

Boris Mironovich Polanuer

A method for manufacturing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity such as (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof using a bacterium transformed with a DNA fragment containing a gene coding for a protein having 2-oxoglutarate-dependent enzyme activity such as L-isoleucine dioxygenase activity; and wherein said bacterium has the ability to produce a product such as (2S,3R,4S)-4-hydroxy-L-isoleucine.

Bacterium producing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity and a method for manufacturing the product

A method for producing shikimic acid, comprising the steps of cultivating a bacterium belonging to the genus Bacillus which is deficient in shikimate kinase activity and has shikimic acid productivity, or a bacterium belonging to the genus Bacillus which is deficient in 5-enolpyruvylshikimate-3-phosphate synthase activity and has shikimic acid productivity, in an medium, producing and accumulating shikimic acid in the medium, and collecting shikimic acid from the medium.

Method for producing shikimic acid

Growing community of inventors

Moscow, Russia

Tatyana Aleksandrovna Bachina