Kikkoman Corporation

Tatsuya Sakakibara

Seiji Murakami

Kazuhiro Imai

Motoo Nakajima

Noriaki Hattori

Teruo Watarai

Keiko Yajitate

Naoki Eisaki

Toshinori Igarashi

Naoki Kajiyama

Eiichi Nakano

Masaru Suzuki

Hiroki Tatsumi

Ayumi Arai

Morisaku Saito

Yasushi Haketa

A method for measuring stains of a test sample by measuring adenosine phosphates. Luminescence of the adenosine phosphates is induced by a luminescent reagent ( ) containing an ATP regenerating enzyme. The level or quantity of luminescence is detected by a silicon photodiode ( ) to thereby measure the stains.

Method and apparatus for measuring sample by luminescence

A wipe inspecting instrument comprising a sample collecting member and a reactive reagent container. The sample collecting member comprises a tubular main body having both upper and lower ends thereof opened, a sample wiping member removably fitted to the upper open portion of the main body and an extracting liquid container disposed below the main body. The sample wiping member includes a retaining member inserted into the upper part of the main body and a cotton swab held by the retaining member. Further, the sample collecting member includes a splash-proofing member disposed at a lower part of the extracting liquid container, for preventing the splash of the extracting liquid. The reactive reagent container has a test tube shape and so inserted into the open portion at the lower end of the main body as to be capable of moving up and down.

Sample collecting member and wiping inspection instrument

The present invention relates to luminescent playthings such as candles, table lights, pen lights, illumination, illuminating playthings for camp, illuminating playthings for night fishing, fish-gathering lamps, safety candles, neon lights, luminescent inks, luminescent pens, luminescent coatings or luminescent writing implements, in which a soft light of an indescribable tone of color not obtainable from flames of conventional candles is emitted via seawater, lake and marsh water, river water, ground water, tap water or mineral drinking water by utilizing 3 components of luciferin, luciferase and ATP or 4 components of these components plus a metal salt, out of components necessary for bioluminescence in fireflies.

Luminescent tool, its auxiliary member and method of preserving bioluminescent composition used in the tool and the auxiliary member

The present invention provides a process for eliminating effectively ATP in a sample, using adenosine phosphate deaminase alone or in combination with at least one enzyme from the group consisting of apyrase, alkaline phosphatase, acid phosphatase, hexokinase and adenosine triphosphatase, a process for determining biological cells contained in foods and beverages in convenient and precise manner in combination with bioluminescence method, and a reagent for the determination thereof.

ATP eliminator and the process for determining biological cells

The present invention provides a process for eliminating effectively ATP in a sample, using adenosine phosphate deaminase alone or in combination with at least one enzyme selected from the group consisting of apyrase, alkaline phosphatase, acid phosphatase, hexokinase and adenosine triphosphatase, a process for determining biological cells contained in foods and beverages in convenient and precise manner in combination with bioluminescence method, and a reagent for the determination thereof.

There is provided a bioluminescence reagent comprising at least pyruvate orthophosphate dikinase, phosphoenolpyruvic acid, pyrophosphoric acid, magnesium ion or another metallic ions, luciferin and luciferase, which reagent is such that the amount of luminescence is maintained in a high level and moreover stably without decaying for a long time in a bioluminescence reaction, and there is provided a method for quantitatively determining an adenosine phosphate ester or a substance taking part in the ATP conversion reaction in high sensitivity and high accuracy using an inexpensive and simple measuring apparatus.

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Noda, Japan

Tatsuya Sakakibara