Kyoto University

Sony Corporation

Central Institute for Experimental Animals

Osaka University

Mitsubishi Tanabe Pharma Corporation

Tatsutoshi Nakahata

Megumu Saito

Akira Niwa

Eriko Matsui

Suguru Dowaki

Naoki Yamamoto

Shiori Oshima

Kiyoshi Ando

Mamoru Ito

Kazuo Sugamura

Koichiro Tsuji

Tatsuji Nomura

Toshio Heike

Kohichiro Tsuji

Feng Ma

Kimio Kobayashi

Sonoko Habu

Yoshio Koyanagi

Kenji Matsumoto

Shinya Yamanaka

Hirokazu Tatsuta

Takeshi Kunihiro

Kiyotoshi Sekiguchi

Hirohisa Saito

Takayuki Tanaka

Masakatsu Yanagimachi

Tomonari Awaya

Ryo Ota

Asako Hatsuyama

Kiminari Ito

An observation system includes a microscope optical system, an image pickup unit, an image pickup control unit, a detection unit, and an observation control unit. The image pickup unit is configured to take an image of a field-of-view range of the microscope optical system. The image pickup control unit is configured to cause the image pickup unit to take images of an observation sample in the field-of-view range at a plurality of focal positions and generate detection images. The detection unit is configured to detect a three-dimensional position of an observation target object in the observation sample from the detection images. The observation control unit is configured to fit the field-of-view range of the microscope optical system to the three-dimensional position.

Observation system, observation program, and observation method

Provided is a method for producing vascular endothelial cells from pluripotent stem cells, the method comprising the following steps (i) to (iii): (i) a step of culturing pluripotent stem cells in a culture medium comprising a BMP, on a culture vessel coated with a first matrix, to produce mesodermal progenitor cells; (ii) a step of dissociating the resulting cells into single cells; and (iii) a step of culturing the resulting cells in a culture medium comprising VEGF, on a culture vessel coated with a second matrix selected from the group consisting of laminin-411 or a fragment thereof, laminin-511 or a fragment thereof, Matrigel, type IV collagen and fibronectin.

Method for inducing vascular endothelial cells

The present invention provides a method for screening drugs for suppressing inflammasome activity, using macrophages derived from induced pluripotent stem cells (iPS cells) having mutant NLRP3 gene.

Method for screening drugs for suppressing inflammasome activity

A dendritic cell is produced from pluripotent stem cells by culturing pluripotent stem cells by the following steps: (1) performing adherent culture in a medium which comprises a BMP family protein but does not comprise serum; (2) performing adherent culture in a medium which comprises VEGF but does not comprise serum; (3) performing adherent culture in a medium which comprises a hematopoietic factor but does not comprise serum; and (4) performing suspension culture in a medium which does not comprise serum.

Method for producing dendritic cells from pluripotent stem cells

The present invention relates to a method for producing human eosinophils from human pluripotent stem cells. More specifically, the present invention provides a method for producing human eosinophils from human pluripotent stem cells, which method comprises the steps of: (1) co-culturing, in the presence of VEGF, human pluripotent stem cells with cells separated from the AGM region of a mammalian fetus; (2) performing suspension culture using a medium comprising IL-3, IL-6, Flt3 ligand, SCF, TPO and serum; (3) performing suspension culture using a medium comprising IL-3, SCF, GM-CSF and serum; and, optionally, (4) performing suspension culture using a medium comprising IL-3, IL-5 and serum.

Method for production of eosinophil from pluripotent stem cell

A method for inducing the differentiation of pluripotent stem cells into skeletal muscle or skeletal muscle progenitor cells is provided. Specifically, a method for producing artificial skeletal muscle or skeletal muscle progenitor cells from human pluripotent stem cells is provided, comprising the following steps of: (1) culturing human pluripotent stem cells by suspension culture; (2) culturing a cell population after suspension culture by adhesion culture; (3) dissociating cells after adhesion culture; and (4) culturing the dissociated cells by adhesion culture. Artificial skeletal muscle or induced skeletal muscle progenitor cells prepared by the method are also provided.

Method for inducing differentiation of pluripotent stem cells into skeletal muscle or skeletal muscle progenitor cells

The present invention relates to a method for producing human mast cells from human pluripotent stem cells. More particularly, the present invention provides a method for producing human mast cells from human pluripotent stem cells, comprising the steps of: (a) culturing human pluripotent stem cells under a condition suitable for promoting differentiation of the human pluripotent stem cells into hematopoietic progenitor cells expressing CD34; and (b) culturing the cells obtained in step (a) in the presence of hematopoietic factors comprising thrombopoietin (TPO) and Flt3 ligand.

Method for producing mast cells from pluripotent stem cells

A blood analysis apparatus includes an analysis section. The analysis section is configured to compare a measurement spectrum obtained regarding red blood cells with a standard spectrum of heme, biliverdin, and bilirubin, and cause the measurement spectrum to belong to any one of the standard spectra.

Blood analysis apparatus and blood analysis method

The present invention provides a method for inducing differentiation of pluripotent stem cells into mesodermal cells, comprising the step of culturing pluripotent stem cells in a serum-free medium without forming an embryoid body and without coculturing with cells from a different species.

Method for producing mesodermal cells by culturing under adherent conditions and without co-culture with cells from a different species in a serum-free medium

The present invention provides an immunodeficient mouse (NOG mouse) suitable for engraftment, differentiation and proliferation of heterologous cells, and a method of producing such a mouse. This mouse is obtained by backcrossing a C.B-17-scid mouse with an NOD/Shi mouse, and further backcrossing an interleukin 2-receptor &#x3b3;-chain gene-knockout mouse with the thus backcrossed mouse. It is usable for producing a human antibody and establishing a stem cell assay system, a tumor model and a virus-infection model.

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The present invention provides useful means in an expansion culture system for a hematopoietic cell (hematopoietic stem cell, hematopoietic progenitor cell). Specifically, the present invention provides a composition for expanding a hematopoietic cell (hematopoietic stem cell, hematopoietic progenitor cell) containing recombinant human serum albumin; a serum-free medium for expanding a hematopoietic cell containing a basal medium and recombinant human serum albumin; a method of expanding a hematopoietic cell comprising culturing a hematopoietic cell in a serum-free medium containing recombinant human serum albumin, and a culture of a hematopoietic cell that can be obtained by the expansion method.

Method of amplifying hematopoietic stem cell and hematopoietic progenitor cell

Method of producing a mouse suitable for the engraftment, differentiation and proliferation of heterologous cells, mouse produced by this method and use of the mouse

Growing community of inventors

Kyoto, Japan

Tatsutoshi Nakahata