Kao Corporation

Tatsuro Ozaki

Hiroyuki Ohta

Koichi Hori

Akihito Kawahara

Shinji Sugihara

Mika Terai

Takeshi Saito

Hiroko Endo

Tadahiro Ozawa

Takuto Tojo

A method of improving photosynthetic ability of an alga, containing enhancing expression of a transketolase and a fructose-1,6-bisphosphate aldolase.

Method of producing lipid

A method of producing lipids, containing the steps of:

A method of producing lipids, comprising the steps of:

A method of producing a long-chain fatty alcohol, containing culturing a microorganism wherein expression of a gene encoding a 3-ketoacyl-ACP synthase and expression of a gene encoding a fatty acyl-CoA reductase are enhanced;

Method of producing fatty alcohol

An acyl-ACP thioesterase consisting of an amino acid sequence of the 115to 274amino acids set forth in SEQ ID NO: 1; an acyl-ACP thioesterase gene encoding the protein; a transformant having the gene; and a method of producing a lipid using the transformant.

Acyl-ACP thioesterase

Provided is a method of producing a lipid, the method comprising the steps of:

[Problems] To provide a method of producing a lipid, containing enhancing productivity of medium chain fatty acids or the lipid containing these medium chain fatty acids as components. [Means to solve] A method of producing a lipid, containing the steps of:

Method of producing lipid using acyl-ACP thioesterase

The present disclosure provides a gene encoding a protein having &#x3b2;-ketoacyl-ACP synthase activity having at least 60% or more identity with the amino acid sequence set forth in SEQ ID NO:1, the protein encoded by the gene, a method of producing a transformant by transforming a host with the gene, a transformant that is transformed with the gene, and a method of producing a lipid, especially, a lipid containing a medium chain fatty acid or ester thereof, by culturing the transformed host that expresses the gene. In some embodiments, the transformant also has a gene encoding an acyl-ACP thioesterase.

Method of producing lipid by using &#x3b2;-ketoacyl-ACP synthase

An acyl-ACP thioesterase consisting of an amino acid sequence of the 72to 233amino acids set forth in SEQ ID NO: 1, an acyl-ACP thioesterase gene encoding the protein, a transformant having the gene, and a method of producing a lipid using the transformant.

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Auburn Hills, MI, United States of America

Tatsuro Ozaki