Hiroshima University

Repertoire Genesis Incorporation

Reperptoire Genesis Incorpration

Tatsuo Ichinohe

Takashi Yamamoto

Tetsushi Sakuma

Ryuji Suzuki

Takahiko Miyama

Takakazu Kawase

Yasuko Honjo

The present disclosure provides a technique whereby the influence of an endogenous TCR is eliminated in TCR gene transfer. A TCR gene is edited using a genome editing enzyme, said genome editing enzyme having one characteristic that amino acids at two specific positions in DNA-binding modules contained in a DNA-binding domain thereof show repeating patterns which differ from one module to another among the four DNA-binding modules. Thus, a lowering in the expression efficiency of the transferred TCR caused by mispairing with an endogenous TCR and the occurrence of a self-reactive TCR are avoided.

Full replacement technique for T cell receptor using platinum TALEN

The present disclosure provides a technique for producing regulatory T cells specific to a desired antigen. Regulatory T cells specific to an antigen are produced by a method that includes: a step for identifying a T cell receptor (TCR) clone present in an effector T cell population specific to an antigen in an effector T cell donor; and a step for introducing all or part of the nucleic acid sequence of the TCR&#x3b1; gene and all or part of the nucleic acid sequence of the TCR&#x3b2; gene included in the clone into a regulatory T cell, said step comprising introducing the TCR&#x3b1; and the TCR&#x3b2; so as to be expressed in pairs.

Technique for creating antigen-specific regulatory T cells (Treg) in which effector T cell (Teff) antigen receptors are used

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Hiroshima, Japan

Tatsuo Ichinohe