Nisshinbo Industries, Inc.

Tatsuo Ichihara

Osamu Suzuki

Gen Masuda

Soichiro Takenishi

Shogo Moriya

Hirohiko Yokomizo

Namiko Nakajima

Kazuko Komiya

Namiko Shiohata

Naokazu Sasaki

Akihisa Urano

Syuiti Abe

Yoshiyuki Matsumura

Sanae Okada

A kit comprising an oligonucleotide-immobilized substrate obtained by immobilizing, on a substrate, one or more kinds of oligonucleotides that enable detection of a polymorphism in a nucleotide sequence of mitochondrial DNA control region of chum salmon, of which standard is represented by the nucleotide sequence of SEQ ID NO: 8, at a position selected from the 10th, 30th, 42nd, 57th, 70th, 96th, 108th, 154th, 194th, 231st, 242nd, 250th, 260th, 339th, 340th, 386th, 395th, 401st and 471st positions is used to detect the polymorphism based on hybridization of the oligonucleotides with a nucleic acid derived from specimen chum salmon and thereby determine a haplotype of the specimen chum salmon.

Method for determining chum salmon haplotype using mitochondrial DNA

A nucleic acid to be immobilized and used for hybridization of nucleic acids using an immobilized nucleic acid, which has a polymer comprising a compound having an unsaturated bond, said polymer being bonded to the 3' end or 5&#x2032; end or both ends of the nucleic acid; a nucleic acid-immobilized substrate comprising a substrate for immobilizing a nucleic acid and the polymer-having nucleic acid immobilized on the substrate; and a method for detecting a nucleic acid by hybridization using an immobilized nucleic acid, which comprises using the nucleic acid-immobilized substrate.

Immobilized nucleic acid and method for detecting nucleic acid

A nucleic acid-immobilized substrate which comprises a carrier comprising a base material and a compound having a carbodiimide group or an isocyanate group carried by the base material, and the same kind or different kinds of nucleic acids immobilized in the form of dots through the carbodiimide group or the isocyanate group at a plurality of sites on the carrier.

Nucleic acid-immobilized substrate

A method is provided, comprising the steps of reacting a biologically active first substance immobilized on a carrier with a second substance capable of specifically binding the first substance, and detecting a non-bound part of the second substance or a bound part of the second substance indirectly bound to the carrier through binding between the first and second substances so that the first substance or the second substance in a sample is analyzed, wherein the carrier carries a compound having 2 to 100 carbodiimide groups, and the first substance is immobilized on the carrier through the carbodiimide groups so that the active substance such as protein and nucleic acid is bound to the carrier conveniently, efficiently, and tightly.

Method for analyzing biologically active substances

The present invention relates to a carbodiimide derivative represented by the following general formula:

Carbodiimide derivative

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Chiba, Japan

Tatsuo Ichihara