Isis Pharmaceuticals, Inc.

Juridical Foundation the Chemo-sero-therapeutic Research Institute

Tatsuo Eto

Chikateru Nozaki

Hiroshi Nakatake

Kevin P Anderson

Ronnie C Hanecak

Fukusaburo Hamada

Tsukasa Nishihara

Kazuya Hoshiko

Shinichi Furukawa

Thomas W Bruice

Walter F Lima

Shoji Furusako

Nobuya Ohtomo

Yoichiro Kino

Keiichi Makizumi

Shoji Furasako

Antisense oligonucleotides are provided which are complementary to and hybridizable with at least a portion of HCV RNA and which are capable of inhibiting the function of the HCV RNA. These oligonucleotides can be administered to inhibit the activity of Hepatitis C virus in vivo or in vitro. These compounds can be used either prophylactically or therapeutically to reduce the severity of diseases associated with Hepatitis C virus, and for diagnosis and detection of HCV and HCV-associated diseases. Methods of using these compounds are also disclosed.

Compositions and methods for treatment of Hepatitis C virus-associated diseases

Compositions and methods for treatment of hepatitis C virus-associated diseases

A novel recombinant plasmid inserted with a herpes simplex virus gene, which comprises a plasmid vector containing a yeast DNA sequence and an Escherichia coli DNA sequence and carrying a promoter region and a herpes simplex virus gN gene (HSVgB) gene) recombined thereto under control of the promoter, said HSVgB gene lacking an N-terminal portion of the gene including a signal sequence-encoding region and optionally further lacking the region downstream therefrom, such as a gB gene lacking a DNA sequence encoding the N-terminal 30 amino acids, and a gB gene lacking a DNA sequence encoding the N-terminal 83 amino acids. The recombinant plasmid is useful for the production of transformed yeast, which is useful for the production of HSVgB proteins suitable for producing HSV vaccine and diagnostic reagents for herpes simplex virus infections.

Recombinant plasmid inserted with herpes simplex virus gene

Growing community of inventors

Ohzu-machi, Japan

Tatsuo Eto