Nard Institute, Ltd.

Hamamatsu-photonics K.k.

Kabushiki Kaisha Nard Kenkyusho

Tatsuhiro Kobashi

Akihiko Kawasaki

Tohru Koike

Makoto Takahagi

Hideo Tsukada

Shingo Nishiyama

Hidekazu Nakao

Takeru Kitashoji

Yumi Nakamasu

The present invention provides a compound represented by formula (I), wherein in formula (I), Rrepresents CH, F, (CH)&#x2014;F, NH&#x2014;(CH)&#x2014;F, O&#x2014;(CH)&#x2014;F or S&#x2014;(CH)&#x2014;F, and n represents an integer of 1 to 3. 

Compound suitable for detection of vesicular acetylcholine transporter

It is intended to provide a method for easily detecting a phosphorylated peptide (protein) in a test sample by using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) which has been conventionally employed in analyzing a protein; a polyacrylamide gel for electrophoresis to be used in the method; a method of producing the gel; and a synthesizing intermediate in producing the gel. The polyacrylamide gel for electrophoresis to be used in the method of the invention is characterized in that at least a part of the structure thereof has a structure represented by the following formula (I); wherein Mrepresents a transition metal ion; and X represents a linker group. 

Polyacrylamide gel for electrophoresis, polyacrylamide gel electrophoresis method using the same, method of producing the same, and acrylamide compound

The present invention provides a method for measuring a surface plasmon resonance, the method enabling easy detection of the existence of a phosphorylated peptide (protein) and determination whether a peptide is phosphorylated or not in biological materials. The present invention also provides a noble metal compound having high capability of coordination to a phosphorylated peptide, and being conveniently usable in the method. A first method for measuring surface plasmon resonance of the present invention comprises: placing a noble metal compound on a bottom face of a prism, irradiating a light to the prism to detect a reflected light, wherein, the noble metal compound has substituents of following formula (I) on a side opposite to a side contacting the prism, and a subject sample is added to a side having the substituent groups (I) in the noble metal compound. [wherein, X represents a linker group]

Method for measuring a surface plasmon resonance and noble metal compound used for the same

Provided are a method for easily detecting phosphorylated peptides, namely, proteins, in samples derived from living organisms or the like, a method for selectively adsorbing the phosphorylated peptides, and compounds that are highly coordinated to the phosphorylated peptides and usable in the methods. The complex compound is represented by the formula: wherein X is a linker moiety, and Y is a labeling group. The compound (I) is highly coordinated to a phosphorylated peptide, and has a labeling group. Accordingly, with use of the compound (I), the phosphorylated peptide can be easily identified.

Method for labeling phosphorylated peptides, method for selectively adsorbing phosphorylated peptides, complex compounds used in the methods, process for producing the complex compounds, and raw material compounds for the complex compounds

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Amagasaki, Japan

Tatsuhiro Kobashi