Illumina Corporation

Tatjana Singer

Ryan Kelley

Eric Hans Vermaas

Chen Zhao

Kevin Eric Wu

Alex So

Han-Yu Chuang

Dwight Baker

Gordon Bean

Jennifer Lococo

The disclosed embodiments concern methods, systems and computer program products for determining sequences of interest using unique molecular indexes (UMIs) that are uniquely associable with individual polynucleotide fragments, including sequences with low allele frequencies or long sequence length. In some implementations, the UMIs include variable-length nonrandom UMIs (vNRUMIs). Methods and systems for making and using sequencing adapters comprising vNRUMIs are also provided.

Universal short adapters with variable length non-random unique molecular identifiers

The disclosed embodiments concern index oligonucleotides configured to identify sources of samples of nucleic acids and methods, apparatus, systems and computer program products for identifying and making the index oligonucleotides. In some implementations, the index oligonucleotides include a set of index sequences, a Hamming distance between any two index sequences of the set of index sequences meeting one or more criteria. System, apparatus, and computer program products are also provided for determining a sequence of interest using the index oligonucleotides.

Universal short adapters for indexing of polynucleotide samples

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San Diego, CA, United States of America

Tatjana Singer