Albert-ludwigs-universitaet Freiburg

University of London

Cellectis

Albert-ludwigs-universitat Freiburg

Tatjana Cornu

Toni Cathomen

Jamal Alzubi

Gleb Sukhorukov

Irina Nazarenko

Marianna Romito

Claudio Mussolino

Yana Tarakanchikova

Valentina Pennucci

Philippe Duchateau

Agnes Gouble

Tafadzwa Mlambo

Sandra Nitsch

Antonio Carusillo

The present invention relates to a biodegradable multilayer nanocapsule for the delivery of at least one biologically active agent into at least one target cell consisting of at least two layers of at least two biodegradable polymers which are laid one onto the other and whereby the biologically active agent is layered onto a layer of a biodegradable polymer and covered with a further layer of a biodegradable polymer, whereby one biologically active agent is a nucleic acid.

Biodegradable multilayer nanocapsules for the delivery of biologically active agents in target cells

The invention pertains to the field of cell therapy and HIV treatments. It provides with highly specific reagents for reducing or inactivating expression of CCR5 in primate and human primary cells, especially under the form of TALE-nucleases. These reagents allow the production of safer primary hematopoietic cells made resistant to HIV, stem cells or differentiated cells, for their infusion into HIV patients.

Sequence specific reagents targeting CCR5 in primary hematopoietic cells

Herein described is a construct for epigenomic modification of genes composed of: (a) a Kr&#xfc;ppel-associated box zinc finger protein or homologous, (b) a DNA region capable of binding to the target gene or homologous, (c) a human DNA methyltransferase DNMT3A or homologous and (d) a murine DNA methyltransferase Dnmt3L or homologous, wherein components a), b), c) and d) are linked to each other either directly or via at least one linker. The construct is a designer epigenome modifier that can be used to silence genes coding for a protein in leukocytes that avoids the internalization of HI viruses in immune cells.

Construct for epigenetic modification and its use in the silencing of genes

Growing community of inventors

Freiburg, Germany

Tatjana Cornu