Siemens Healthcare Diagnostics Gmbh

Tatiana Mareeva

Zhu Teng

Manoj Sharma

Tie Q Wei

William D Bedzyk

Yuriy Smirnov

Yi Feng Zheng

Martin A Drinan

Jie Li

Izak Bahar

Nihmat Morjana

Bogdan Draghici

Tatiana Lebedeva

Heungyeung Yau

Allison Dominowski

Tie Quan Wei

Antibodies that specifically bind naloxone or naltrexone are disclosed. Also disclosed are conjugates used in the production of the antibodies, as well as methods of producing the antibodies. Also disclosed are methods of using the antibodies in direct assays for naloxone or naltrexone. Further disclosed are methods of reducing naloxone or naltrexone interference in opiate assays using the antibodies.

Anti-naloxone and anti-naltrexone monoclonal antibodies and methods of production and use thereof

Antibodies are raised against compounds of Formula (I) where Z is an immunogenic carrier. These compounds are capable of binding specifically to corresponding antibodies. The antibodies are specific for epimers of vitamin D. Antibodies in accordance with the principles described herein may be employed to minimize or eliminate 3-epimer cross-reactivity in assays for non-epimeric forms of vitamin D analytes. Over-estimation of total non-epimeric vitamin D analyte caused by the cross-reactivity of 3-epimer vitamin D with an antibody for vitamin D analyte can be substantially avoided employing, as blocking agents, antibodies prepared against immunogens that are a compound of the Formula (I) wherein Z is an immunogenic carrier. 

Binding partners specific for vitamin D epimers

Methods include determining an amount of an epimeric vitamin D analyte in a sample suspected of containing the epimeric vitamin D analyte. A combination is provided in an assay medium that includes the sample and a vitamin D epimer antibody that is specific for the epimeric vitamin D analyte. The assay medium is incubated under conditions for binding of the vitamin D epimer antibody to the epimeric vitamin D analyte to form an epimeric vitamin D antibody-bound complex. The amount of the epimeric vitamin D antibody-bound complex is determined and related to the amount of epimeric vitamin D analyte in the sample.

Assays for vitamin D epimers

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Haddonfield, NJ, United States of America

Tatiana Mareeva