Kyoto University

Orizuru Therapeutics, Inc.

Taro Toyoda

Kenji Osafune

Yasushi Kondo

Kazuwa Nakao

Junji Yamaura

Shuhei Konagaya

Nobuya Inagaki

Noriko Yamazoe

Azuma Kimura

Masakatsu Sone

Shinobu Kuwae

Hideyuki Hiyoshi

Taisuke Mochida

Fumihiko Shiota

Daisuke Taura

An object of the present invention is to provide a novel method for producing a biological tissue-like structure comprising differentiated cells induced from pluripotent stem cells, and the present invention provides a method of forming a biological tissue-like structure together with host-derived blood vessels and connective tissue by transplanting a composition in which cells derived from pluripotent stem cells are disposed to be dispersed in a biocompatible material to induce the differentiation of the cells.

Method for producing biological tissue-like structure

Provided is a cell suspension culturing apparatus suitable for large-scale culture of cells, the cell culturing apparatus comprising a culture solution aspirator having a double-tube structure comprising an outer tube and an inner tube inserted in a lumen of the outer tube, and a suspension culture vessel, wherein in the culture solution aspirator, the outer tube comprises a filter through which a culture solution is passed, the inner tube comprises a suction port for the culture solution passed through the filter, and an air hole which communicates the lumen of the outer tube with the outside is disposed distally in a direction of insertion of the outer tube into the suspension culture vessel.

Cell culturing apparatus, culture solution aspirator, and cell culturing method

Provided is a method for generating pancreatic bud cells, having the step of culturing PDX1/NKX6.1cells in a medium containing KGF, EGF and a BMP inhibitor. The culturing step may be performed in suspension cultures or in adherent cultures. When the cells are cultured in adherent cultures, the cells may be cultured in a medium further containing a ROCK inhibitor or a nonmuscle myosin II inhibitor.

Method for generating pancreatic bud cells and therapeutic agent for pancreatic disease containing pancreatic bud cells

Provided is a method for inducing pancreatic hormone-producing cells from pancreatic progenitor cells efficiently. The method comprises a step of culturing the cells in a culture medium comprising sodium cromoglicate.

Method for generating pancreatic hormone-producing cells

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Kyoto, Japan

Taro Toyoda