10x Genomics, Inc.

Tarjei Sigurd Mikkelsen

Katherine Pfeiffer

Serge Saxonov

John R Stuelpnagel

Michael Ybarra Lucero

Paul Ryvkin

Josephine Harada

Andrew D Price

Phillip Belgrader

Zachary Bent

Jessica Michele Terry

Michael Schnall-Levin

Christopher Hindson

Elliott Meer

Stephane Claude Boutet

Christopher Joachim O'Keeffe

Jonas Frisen

Patrik Stahl

Vijay Kumar Sreenivasa Gopalan

Solongo B Ziraldo

Geoffrey McDermott

Eswar Prasad Ramachandran Iyer

Mohammad Rahimi Lenji

Andrew Kohlway

Luigi Jhon Alvarado Martinez

Maja Marklund

Enric Llorens Bobadilla

Benjamin J Hindson

Rajiv Bharadwaj

Tobias Daniel Wheeler

Indira Wu

Niranjan Srinivas

Sarah Taylor

Jason Underwood

Patrick Marks

David Jaffe

The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample

Provided herein are compositions, systems and methods for tagging molecular events, reactions, species, etc., but without the need for complex, highly diverse libraries of tagging molecules. Provided are tagging moieties that can have a smaller number, a few, or even a single original 'tagging' structure that may be transformed or transformable, in situ, into a collection of larger numbers of unique tagging or “barcode” moieties.

Systems and methods for differentially tagging nucleic acid molecules

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for 'tagging' analytes for characterization.

Methods and systems for processing polynucleotides

The present disclosure provides methods of processing or analyzing a sample. A method for processing a sample may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule), barcoding the probe-nucleic acid molecule complex, and performing extension, denaturation, and amplification processes. A method for processing a sample may comprise hybridizing first and second probes to adjacent or non-adjacent target regions of a nucleic acid molecule (e.g., an RNA molecule), linking the first and second probes to provide a probe-linked nucleic acid molecule, and barcoding the probe-linked nucleic acid molecule. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well. One or more processes of the methods described herein may be performed on a cell, such as a permeabilized cell.

Methods and systems for characterizing analytes from individual cells or cell populations

The present disclosure provides methods and systems for producing full-length sequencing information of transcriptomes from single cells or from the bulk. Random ligation and circularization of barcoded or non-barcoded complementary deoxyribonucleic molecules can be used to provide a circular template for amplification and subsequent sequencing.

Transcriptome sequencing through random ligation

Provided herein are compositions, systems and methods for tagging molecular events, reactions, species, etc., but without the need for complex, highly diverse libraries of tagging molecules. Provided are tagging moieties that can have a smaller number, a few, or even a single original 'tagging' structure that may be transformed or transformable, in situ, into a collection of larger numbers of unique tagging or 'barcode' moieties.

Systems and methods for nucleic acid processing using degenerate nucleotides

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Pleasanton, CA, United States of America

Tarjei Sigurd Mikkelsen