Triad National Security, LLC

Ut-Battelle, Inc.

University of Georgia Research Foundation Inc

Alliance for Sustainable Energy, LLC

Taraka Dale

Ramesh K Jha

Gregg Tyler Beckham

Christopher W Johnson

Adam Michael Guss

Charlie E Strauss

Ellen Lee Neidle

Joshua R Elmore

Gayle Joann Bentley

George Lee Peabody, V

Niju Narayanan

Peter Corbin St John

Ryan E Bermel

Davinia Salvachua Rodriguez

Biosensors for detection terephthalic acid (TPA) and methods of their use are provided. The biosensors include a nucleic acid encoding a TphR protein, a promoter regulated by TPA, and a reporter operably linked to the promoter. Vectors and host cells including the biosensors are also provided.

Terephthalate biosensors and uses thereof

Biosensors including a nucleic acid encoding a PcaU protein, a PobR protein, a CatM protein, a PcaR protein, or a TphR protein are provided. In some examples, the biosensors include a promoter regulated by the sensed molecule operably linked to a reporter gene. The biosensors may be included in a vector or in cells including one or more of the biosensors or vectors. Modified chorismate pyruvate lyase (UbiC) and modified paraoxonase (PON1) proteins including one or more amino acid substitutions are provided. Finally, methods of selecting biocatalysts with increased activity including transforming a library of cells expressing a biosensor with one or more nucleic acids encoding one or more mutations in a gene involved in a biosynthesis pathway, determining activity of the reporter protein; and selecting a cell with increased reporter protein activity as expressing a biocatalyst with increased activity are provided.

Modified biosensors and biocatalysts and methods of use

The present disclosure relates to a non-naturally occurring microorganism that includes an endogenous genetic deletion that eliminates the expression of at least a pyruvate kinase, where the genetically modified prokaryotic microorganism is capable of producing 3-deoxy-D-arabino-heptulosonate-7-phosphate.

Engineered microorganisms for the production of intermediates and final products

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Los Alamos, NM, United States of America

Taraka Dale