University of Florida Research Foundation, Incorporated

Georgia Tech Research Corporation

Tapomoy Bhattacharjee

Wallace Gregory Sawyer

Thomas Ettor Angelini

Brent S Sumerlin

Steven Craig Ghivizzani

Carl David Crane

Alberto Fernandez-Nieves

Samantha M Marquez

Ya-Wen Chang

Kyle D Schulze

Curtis Taylor

Kyle Gene Rowe

Glyn Daniel Palmer

Christopher S O'Bryan

Joshua Muse

Meghan Hughes

Cameron Morley

A three-dimensional cell growth medium is described. The cell growth medium may comprise hydrogel particles swollen with a liquid cell growth medium to form a granular gel yield stress material which undergoes a phase transformation from a solid phase to a liquid-like phase when an applied stress exceeds the yield stress. Cells may be placed in the three-dimensional cell growth medium according to any shape or geometry, and may remain in place within the three-dimensional cell growth medium.

Growth media for three-dimensional cell culture

Described herein are various embodiments of a valve that may be opened and closed using a thixotropic or 'stress yield' material, or other material that temporarily changes phase upon application of energy to the material. More particularly, some embodiments may include a valve that is opened and closed using a granular gel that is a temporary phase change material.

Valve incorporating temporary phase change material

Disclosed herein is a bioreactor system that allows perfusive flow through a porous support medium enabling 3D growth of biological samples. In some embodiments, the system comprises a sample well filled with a three-dimensional (3D) cell growth medium. The system can further comprises a liquid medium reservoir fluidly connected to the sample well by a first filter material. The system can further comprises a medium collection chamber fluidly connected to the sample well by a second filter material. The system can further comprise an absorbant material that creates an osmotic pressure gradient to produce perfusive flow. In some embodiments, osmotic pressure draws fluid from the liquid medium reservoir, through the first filter material, into the sample well where it permeates the three-dimensional cell growth medium, through the second filter material, and finally into the medium collection chamber.

Perfusion bioreactor driven by osmotic pressure gradients

A method or apparatus for creating a three-dimensional tissue construct of a desired shape for repair or replacement of a portion of an organism. The method may comprise injecting at least one biomaterial in a three-dimensional pattern into a first material such that the at least one biomaterial is held in the desired shape of the tissue construct by the first material. The apparatus may comprise an injector configured to inject at least one biomaterial in a three-dimensional pattern into a first material such that the at least one biomaterial is held in the desired shape of the tissue construct by the first material. The first material may comprise a yield stress material, which may be a material exhibiting Herschel-Bulkley behavior. The tissue construct may have a smallest feature size of ten micrometers or less.

High speed 3D printing system for wound and tissue replacement

An organic microgel system as support material for 3D printing of soft materials such as silicone and methods for manufacturing and using the organic microgel system are disclosed. In some embodiments, the organic microgel system comprises a plurality of microgel particles formed by blending a di-block copolymer and a tri-block copolymer in an organic solvent. The organic microgel system may allow high precision 3D printing of silicone objects with complex shapes.

Organic microgel system with di&#x2014;and tri-block blends of herschel-bulkley fluid for 3D printing of sacrificial support structures

Organic microgel system for 3D printing of silicone structures

Growing community of inventors

Gainesville, FL, United States of America

Tapomoy Bhattacharjee