E.i. Dupont De Nemours and Company

Tao Wang

Rick Weizhang Ye

Stephen K Picataggio

John E Seip

Hao Ni

Deana J Dicosimo

James M Odom

Siqun Wang

Mattheos Koffas

Laura Anne Bedzyk

Genes have been identified in thegenome that are responsive to various metabolic and growth conditions. The identified responsiveness of these genes allows for the use of their promoters in regulated gene expression in C1 metabolizing bacteria. In particular, the hps promoter, which in its native state drives the expression of 3-hexulose-6-phosphate synthase (HPS), was found to be useful for directing expression of heterolgous coding regions (e.g., crtZ) in the obligate methanotrophsp. 16a.

Natural promoters for gene expression in C1 metabolizing bacteria

Genes have been identified in the Bacillus genome that are responsive to various metabolic conditions and growth cycle changes. The new responsiveness of these genes allows for their use in regulated gene expression in Bacillus sp. and for the monitoring of bioreactor health.

Natural promoters for gene expression and metabolic monitoring in bacillus species

Genes have been isolated from a Methylomonas sp encoding elements of the exopolysaccharide biosynthetic pathway. The genes and gene products are the first isolated from an organisms capable of utilizing single carbon (C1) substrates as energy sources. The genes are useful for engineering other C1 utilizing microorganisms to make altered levels of exopolysaccharide which is used in a variety of commercial applications.

Genes encoding exopolysaccharide production

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Hockessin, DE, United States of America

Tao Wang