Other

Chinese Academy of Agricultural Sciences

Ge Medical Systems Global Technology Company, LLC

Institute of Animal Science of Chinese Academy

Tao Tu

Jie Zhang

Yuan Wang

Bin Yao

Huoqing Huang

Huiying Luo

Yaru Wang

Yingguo Bai

Xiaoyun Su

Kun Meng

Weina Liu

Rui Ma

Fei Gao

Xia Shi

Yuan Gu

Jin Qiu

Yujie Guo

Jianzhong Ge

Yeqing Li

Huang Qin

Jianshuang Ma

Xiao Jiang

The present invention relates to the field of genetic engineering, particularly to a recombinant expression vector for rapidly screening the high expression strains and a method for rapidly screening high expression strains. In the invention, an exogenous red fluorescent protein andcell surface protein localization signal are fused and expressed, and the fusion gene (DsRed-AfMP1) is integrated into the genome of, so as to construct a strain displaying red fuorescent protein on the surface of. By sortingstrains with red fluorescent protein on the surface by flow cytometry, genes beneficial to the improvement of cellulase activity can be quickly isolated.

Recombinant expression vector applicable to rapid screening for recombinant strain and application

The present invention relates to the field of genetic engineering, particularly to a glucoamylase TIGa15, gene and application thereof. Said glucoamylase comprises the amino acid sequence of SEQ ID NO:1 or SEQ ID NO:2 and has the excellent enzymic properties, which can be applied to feed, food, and medicine industries, can be industrially produce with the genetic engineering technics.

Glucoamylase TLGA15 and gene and application thereof

The present invention provides use of a manganese peroxidase in the detoxification of mycotoxins, and specifically, the present invention provides five manganese peroxidases (MnP-1, MnP-2, MnP-4, MnP-5, and MnP-6), genes thereof, and uses thereof. The present invention provides five manganese peroxidases (MnP-1, MnP-2, MnP-4, MnP-5, and MnP-6) derived from lignocellulose degradation bacteria, the amino acid sequences thereof being as set forth in SEQ ID NO: 1, SEQ ID NO: 4, SEQ ID NO: 7, SEQ ID NO: 10, and SEQ ID NO: 13.

Manganese peroxidase, gene thereof, and use thereof in detoxification of mycotoxin

The present invention takes the glucose oxidase GOD fromas the mutation template to obtain the glucose oxidase GOD mutants with improved catalytic efficiency and thermal stability by site directed mutagenesis. The specific activity of the mutant of the present invention is 66% higher than that of the wild type GOD; the enzyme activity of the mutant of the present invention is 13.6 times higher than that of the wild type after being treated at 70&#xb0; C. for 10 min; and the enzyme activity of the mutant of the present invention is 29.4 times higher than that of the wild type after being treated at 80&#xb0; C. for 2 min.

Glucose oxidase GOD mutant and gene and application thereof

The present invention relates to a mannanase PMan5A mutant having improved heat resistance, a gene encoding the mutant and application thereof. The mutant is obtained by a substitution of histidine with tyrosine at amino acid residue 93, phenylalanine with tyrosine at amino acid residue 94, leucine with histidine at amino acid residue 356, and/or alanine with proline at amino acid residue 389. The thermal tolerance of the single site mutation mutant H93Y, L356H and A389P are greatly improved over that of the wild-type mannanase PMan5A, and the thermal tolerance of the combination mutants shows the stack effect of the single site mutation, demonstrating the amino acids at the sites of 93, 94, 356, and 389 play an important role for the thermal stability of the mannanase of GH5 family.

Mannanase PMAN5A mutant having improved heat resistance, gene thereof, and application

The present invention relates to the field of agriculture biotechnology, specially relates to an amylase mutant having high specific activity and thermal stability, gene and use thereof. Said amylase mutant is obtained by performing substitution of S33A/S34E/V35H, and deletion of amino acids at the sites of 178 and 179 of the wild type amylase having amino acid sequence of SEQ ID NO:1, and having improved enzymatic activity and thermal stability than the wild type amylase.

Amylase mutant having high specific activity and thermal stability, gene of mutant, and applications thereof

Provided are acidic thermophilic polygalacturonase TePG28A, encoding gene and application thereof. The amino acid sequence thereof is as shown in SEQ ID NO. 1 or SEQ ID NO. 2. The expressed acidic thermophilic polygalacturonase by means of cloning has advantages such as high enzyme activity and high stability; can adapt to the high temperature environment in the industrial production; has better application prospect; and can effectively degrade pectic substances such as polygalacturonic acid and pectin; and can be effectively applied to the industrial field of feed, food, and textile, etc.

Acidic thermophilic polygalacturonase TEPG28A, and encoding gene and application thereof

Provided are a glucose oxidase CnGODA, an encoding gene thereof, a recombinant expression vector comprising the gene, and a recombinant strain; the amino acid sequence of the glucose oxidase CnGODA is as represented in SEQ ID NO:1 or SEQ ID NO:2. Further provided is a method for use in preparing glucose oxidase CnGODA, and application of glucose oxidase CnGODA.

Glucose oxidase CnGODA and gene and application thereof

An X-ray CT apparatus permitting compatibility between the resolution orientation and the coverage orientation at low cost is to be realized. The X-ray CT apparatus uses as the X-ray detector what has a former half channel part and a latter half channel part which are the same in the number of slices and differ in the coverage in the body axis direction of the subject and in slice thickness, and selectively performs scan control in the high resolution mode in which the coverage and the slice thickness are smaller and scan control in the high coverage mode in which the coverage and the slice thickness are larger.

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Beijing, China

Tao Tu