Cargill Inc.

Lynch, Michael

Copley, Shelley

Louie, Michael

Tanya Lipscomb

Hans H Liao

Eileen Spindler

Wendy Kathleen Ribble

Michael David Lynch

Michael Tai Man Louie

Travis Robert Wolter

Christopher Patrick Mercogliano

Steffen Schaffer

Liv Reinecke

Katrin Grammann

Jan Wolter

Catherine B Poor

Brittany Prather

Ronald J Evans

Dave A Hogsett

Ron Evans

Shelley Copley

Matthew Lipscomb

Brittany Robinson

Michael Louie

David A Hogsett

Dave Hogsett

The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.

Acetyl-CoA carboxylases

A microbial cell is used for producing at least one fatty acid ester, wherein the cell is genetically modified to contain (i) at least one first genetic mutation that enables the cell to produce at least one fatty acid and/or acyl coenzyme A (CoA) thereof by increased enzymatic activity in the cell relative to the wild type cell of malonyl-CoA dependent and malonyl-ACP independent fatty acyl-CoA metabolic pathway, wherein the fatty acid contains at least 5 carbon atoms; and (ii) a second genetic mutation that increases the activity of at least one wax ester synthase in the cell relative to the wild type cell and the wax ester synthase has sequence identity of at least 50% to a polypeptide of SEQ ID NO: 1-8 and combinations thereof or to a functional fragment of any of the polypeptides for catalyzing the conversion of fatty acid and/or acyl coenzyme A thereof to the fatty acid ester.

Production of fatty acids esters

This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a fatty acid or fatty acid derived product, wherein the modified microorganism produces fatty acyl-CoA intermediates via a malonyl-CoA dependent but malonyl-ACP independent mechanism.

Microorganisms and methods for the production of fatty acids and fatty acid derived products

Bioproduction of chemicals

Acetyl-coA carboxylases

Growing community of inventors

Boulder, CO, United States of America

Tanya Lipscomb