Amano Pharmaceutical Co., Ltd.

Ajinomoto Co., Ltd.

The Japanese Research & Development Association for Bioreactor System in

Tamio Mase

Shotaro Yamaguchi

Takaichi Ohya

Narihide Matsuzaki

Jun Kurashige

Nobumasa Yokoi

Satoru Asada

This invention relates to a .gamma.-cyclodextrin glucanotransferase having novel properties, to a process for the production of the .gamma.-cyclodextrin glucanotransferase which comprises culturing a strain belonging to the genus Brevibacterium capable of producing cyclodextrin glucanotransferase, thereby allowing the strain to produce the .gamma.-cyclodextrin glucanotransferase in a culture medium, and subsequently collecting the enzyme, to a process for the production of cyclodextrin which comprises allowing the cyclodextrin glucanotransferase to react with substrate dissolved in a solution, thereby effecting formation of .gamma.-cyclodextrin as a main product, and to a method for increasing .gamma.-cyclodextrin yield without accompanying yield increase of total cyclodextrin, which comprises adding ethyl alcohol to a reaction solution in which .gamma.-cyclodextrin and .beta.-cyclodextrin are formed from starch by the action of .gamma.-cyclodextrin glucanotransferase, thereby effecting repressed formation of .beta.-cyclodextrin and enhanced formation of .gamma.-cyclodextrin. Development of the process of this invention has rendered possible low cost production of .gamma.-cyclodextrin and therefore expands practical use of .gamma.-cyclodextrin to the field of food and the like.

Process of making cyclodextrin glucanotransferase with brevibacterium

A glyceride produce free from triglycerides and containing mostly monoglycerides is made by mixing glycerol and fatty acids with a lipase which is isolated from Penicillium cyclopium ATCC 34613.

Preparation of glycerides having a high content of monglycerides with a

A process for modifying fats and oils which comprises applying partial glyceride lipase to fats and oils containing partial glycerides and fatty acids to synthesize triglycerides from the partial glycerides by esterification. The lipase is preferably an immobilized lipase and this process is preferably carried out under a water concentration of 1500 ppm or less. By using partial glyceride lipase according to the present invention, there can be obtained modified fats and oils having a low content of diglycerides without changing triglyceride composition.

Process for modifying fats and oils

The present invention relates to a method for reforming fats and oils which comprises treating the fats and oils containing partial glycerides with two or more kinds of lipases which are different in a fatty acid specificity and/or a position specificity in the presence of a small amount of water to obtain fats and oils containing partial glycerides in a low content in a high yield. According to the invention, a reaction rate and a yield are improved.

Method for reforming fats and oils with enzymes

Aeromonas sp. No. 212 is cultured to allow it to produce a novel cellulase 212 and/or novel hemicellulase in a culture medium, and the so produced cellulase 212 and/or hemicellulase 333 is separated from the culture medium. The so separated novel cellulase 212 and/or novel hemicellulase 333 is used as a cellulosic material-decomposing agent in purifying tanks, plants for treatment of industrial waste waters, excrement treatment plants, drainage disposal treatment plants and the like.

Growing community of inventors

Ichinomiya, Japan

Tamio Mase