Epigenomics Ag

Tamas Rujan

Kurt Berlin

Manfred Schmitt

Fabian Model

Ralf Lesche

Juergen Distler

Dimo Dietrich

Andrew Z Sledziewski

Jörn Lewin

Nadia Harbeck

Oliver Hartmann

Sabine Maier

Peter Adorjan

Thomas Koenig

Christian Piepenbrock

John W Martens

Armin Schmitt

Jürgen Distler

Heinz Hoefler

John Foekens

Anne Fassbender

Almuth Marx

Inko Nimmrich

Ina Schwope

Maxime P Look

Thomas König

Heinz Höfler

Cathy Lofton-Day

John Martens

Volkmar c/o Department Of Gynecology Müller

The present invention relates to modified and genomic sequences, to oligonucleotides and/or PNA-oligomers for detecting the cytosine methylation state of genomic DNA, as well as to a method for predicting the response of a subject with a cell proliferative disorder of the breast tissues, to endocrine treatment.

Method for determining risk of relapse of breast cancer following tamoxifen adjuvant therapy

Particular aspects relate to a method for determining the methylation pattern of a polynucleic acid, comprising: a) preparing a solution comprising a mixture of fragments of the polynucleic acid; b) coupling the fragments with a substance being detectable with a detection method; c) contacting a solution comprising the fragments of b) with a DNA microarray having a plurality of different immobilized oligonucleotides, each comprising at least one methylation site, at respectively assigned different locations thereon, the contacting under conditions affording hybridization of fragments with correlated immobilized oligonucleotides under defined stringency, and wherein the immobilized oligonucleotides have a length of less than 200 bases; d) optionally performing a a washing step; and e) detecting, using the physical detection method, such immobilized nucleic acids to which solution fragments are hybridized and/or to which solution fragments are not hybridized.

Method for determining the methylation pattern of a polynucleic acid

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Berlin, Germany

Tamas Rujan