Argos Therapeutics, Inc.

Kyowa Hakko Kirin Co., Limited

Tamara Monesmith

Lois Dinterman

Irina Tcherepanova

Rebecca Pogue-caley

Tamara Tatroe Monesmith

Rebecca Pogue-Caley

Richard Alexander Grant

Ian S Fitzpatrick

Arjun Vinoo Caprihan

Christopher Thomas Ryan

Christopher John Leigh-Lancaster

Rebecca Pogue

Centrifuge vessels suitable for live cell processing include a bowl with a cap, and a tube inside the bowl extending between the cap and a lower portion of the bowl. The bottom of the bowl can have a closed annular ring surrounding a center mound. The vessels can be particularly suitable for processing low volumes of live cells for vaccines or other therapies.

Centrifuge vessels with a center mound and an internal elongate tube suitable for live cell processing and associated systems and methods

Methods are provided for the production of dendritic cells from monocytes that have been incubated at a temperature of 1&#xb0; C.-34&#xb0; C. for a period of approximately 6 to 96 hours from the time they are isolated from a subject. After the incubation period, the monocytes can then be induced to differentiate into dendritic cells. Mature dendritic cells made by the methods of the invention have increased levels of one or more of CD80, CD83, CD86, MHC class I molecules, or MHC class II molecules as compared to mature dendritic cells prepared from monocytes that have not been held at 1&#xb0; C.-34&#xb0; C. for at least 6 hours from the time they were isolated from a subject. Dendritic cells made by the methods of the invention are useful for the preparation of vaccines and for the stimulation of T cells.

Dendritic cell compositions and methods

Growing community of inventors

Durham, NC, United States of America

Tamara Tatroe Monesmith