Lonza Cologne Gmbh

Tamara Grabek

Ludger Altrogge

Gregor Siebenkotten

Gudula Riemen

Juliana Helfrich

Elke Lorbach

Helmut Brosterhus

Rainer Christine

Hanns-Martin Schmidt

Dietmar Lenz

Katharina Hein

Marion Gremse

Tatjana Males

Bodo Ortmann

Andrea Mueller-Hartmann

The present invention relates to a method for transfection of cells using at least one protein capable of forming nucleoprotein filaments, wherein the protein is initially modified with at least one functional component which influences one or more steps of the transfection, the nucleic acid to be transfected is then loaded with the modified protein, whereby the nucleic acid and the protein form a filament-like complex, and this complex is finally added to the cells to be transfected. The invention further relates to a transfection agent consisting of nucleoprotein filaments (NPF), with at least one nucleoprotein filament-forming protein being modified with at least one functional component for the transfection. Furthermore, the present invention relates to the use of the transfection agent according to the invention for producing a drug for gene therapeutic treatment of humans and animals. The present inventions also includes corresponding pharmaceutical preparations, especially for use in gene therapy as well as the use of such transfection agents as component in kits.

Modular transfection systems

Growing community of inventors

Wiehl, Germany

Tamara Grabek