National Institute of Agrobiological Sciences

National Agriculture and Food Research Organization

Takuya Tsubota

Hideki Sezutsu

Taiyo Yoshioka

Tsunenori Kameda

Takao Suzuki

Keiro Uchino

Naoyuki Yonemura

Kenichi Nakajima

Tetsuya Iizuka

Hiromitsu Tanaka

A method of producing a transgenic silkworm that spins bagworm silks and producing a large quantity of bagworm silks by transgenic technology is developed and provided. A gene encoding a modified bagworm Fib H and a transgenic silkworm in which the gene is introduced, wherein the gene is obtained by cloning a gene fragment encoding a bagworm Fib H-like polypeptide comprising a partial amino acid sequence of bagworm Fib H, and fusing the gene fragment to a gene fragment encoding silkworm-derived Fib H, are provided.

Recombinant bagworm silk

This invention is intended to develop a promoter that can strongly induce marker gene expression throughout an embryo, so as to simply, efficiently, and accurately identify a transgenic insect at an early developmental stage, and to provide a gene expression vector into which such promoter has been incorporated as a transformant discrimination marker. Such exogenous gene expression vector comprises a polynucleotide comprising the nucleotide sequence as shown in SEQ ID NO: 1 as a promoter.

Exogenous gene expression vector, transformant discrimination marker, and transformant

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Ibaraki, Japan

Takuya Tsubota