Micro Biopharm Japan Co., Ltd.

B Food Science Co., Ltd.

Takumi Tochio

Keisuke Tamura

Tadashi Fujii

Saki Nakamura

Naomi Ito

Misa Yahara

Yoshimi Fukatani

To provide an improved &#x3b2;-fructofuranosidase which is capable of efficiently producing kestose while inhibiting the production of nystose. This improved &#x3b2;-fructofuranosidase comprises either: an amino acid sequence (a) obtained by introducing, into the amino acid sequence represented by SEQ ID NO: 2, an amino acid mutation i) in which the 85th glycine (G) from the N-terminal is substituted for a protein-constituting amino acid other than glycine (G), and/or an amino acid mutation ii) in which the 310th histidine (H) from the N-terminal is substituted for lysine (K), arginine (R), or tyrosine (Y); or an amino acid sequence (b) which exhibits &#x3b2;-fructofuranosidase activity, and which comprises an amino acid sequence obtained by deleting, substituting, inserting, or adding one or more amino acids in (a) other than the amino acid into which the amino acid mutation has been introduced.

&#x3b2;-fructofuranosidase

An improved &#x3b2;-fructofuranosidase is provided. The improved &#x3b2;-fructofuranosidase may comprise an amino acid sequence having 60% or higher identity to the amino acid sequence of SEQ ID NO: 2, and may contain an amino acid mutation that replaces histidine (H) corresponding to position 395 counted from the N terminus of SEQ ID NO: 2 with arginine (R) or lysine (K), an amino acid mutation that replaces leucine (L) at position 123 counted from the N terminus of SEQ ID NO: 2 with cysteine (C), and/or an amino acid mutation that replaces phenylalanine (F) at position 473 counted from the N terminus of SEQ ID NO: 2 with tyrosine (Y).

[Problem] To provide a production method capable of simply and efficiently producing a fructose-added carbohydrate using &#x3b2;-fructofuranosidase. [Solution] A method for producing a fructose-added carbohydrate, said method having a step in which a receptor substrate and a hydrate containing terminal fructose residue are brought into contact with:

Growing community of inventors

Chita, Japan

Takumi Tochio