Ajinomoto Co., Ltd.

Fujitsu Corporation

Takuji Ueda

Mikhail Markovich Gusyatiner

Elvira Borisovna Voroshilova

Konstantin Vyacheslavovich Rybak

Tatyana Viktorovna Leonova

Yuji Joe

Yury Ivanovich Kozlov

Yoshihiro Usuda

Kazuhiko Matsui

Yuta Nakai

Yoshihiko Hara

Vera Georgievna Doroshenko

Sergei Vladimirovich Mashko

Elena Vitalievna Klyachko

Aleksandra Yurievna Skorokhodova

Shintaro Iwatani

Ekaterina Aleksandrovna Slivinskaya

Ekaterina Alekseevna Savrasova

Larisa Gotlibovna Airikh

Stephen Van Dien

Valeriy Zavenovich Akhverdian

Yuichiro Tsuji

Tomoko Suzuki

Mika Moriya

Yoshiya Gunji

Vitaly Grigorievich Paraskevov, Legal Representative

Rie Takikawa

Yuri Nagai

Saori Kataoka

Hisao Ito

Yoko Asakura

Kazuyuki Hayashi

Chie Koseki

Hidetaka Doi

Yusuke Hagiwara

Vitaly Grigorievich Paraskevov

Yuuta Nakai

Yuki Yano

Yuichi Nakahara

There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.

Method for producing L-amino acids using bacteria of the enterobacteriaceae family

An L-amino acid is produced by culturing a microorganism belonging to the family Enterobacteriaceae having an L-amino acid-producing ability and modified so that glycerol dehydrogenase and dihydroxyacetone kinase activities are increased, in a medium containing glycerol as a carbon source to produce and accumulate an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.

Method of producing L-amino acid

L-amino acids are produced by culturing a microorganism which has an ability to produce the L-amino acid, but has been modified so that expression of the ybjE gene has been enhanced. The L-amino acid is collected from the culture medium or from the microorganism.

L-amino acid-producing microorganism and method for producing L-amino acid

A method for producing an L-amino acid is provided which includes culturing in a medium a microorganism of the Enterobacteriaceae family which has an ability to produce an L-amino acid and which has been modified so as to enhance the &#x3b2;-glucoside PTS activity, and collecting the L-amino acid from the medium or cells.

L-amino acid-producing bacterium and a method for producing L-amino acids

An L-amino acid is produced by culturing a microorganism which belongs to the family Enterobacteriaceae and is able to produce an L-amino acid, wherein the bacterium has been modified to enhance orotate phosphoribosyltransferase activity is enhanced, in a medium to produce and cause accumulation of an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.

L-amino acid-producing microorganism and a method for producing an L-amino acid

The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genusor, which has been modified to attenuate expression of a gene coding for sRNA.

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A bacterium belonging to the genuswhich has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.

Method for producing L-lysine or L-threonine

Method for producing L-amino acids using bacteria of the Enterobacteriaceae family

A method for producing an L-amino acid is provided which includes culturing in a medium a microorganism of the Enterobacteriaceae family which has an ability to produce an L-amino acid and which has been modified so as to enhance the mannose PTS activity, accumulating the L-amino acid in the medium or in cells, and collecting the L-amino acid from the medium or cells.

An L-amino acid is produced by culturing an L-amino acid-producing bacterium which belongs to the Enterobacteriaceae family and which has been modified so that the acetyl-CoA synthetase activity is increased.

Method for producing L-amino acid

A metabolic flux affecting substance production using cells is determined by 1) creating a stoichiometric matrix based on formulas of biochemical reactions from a substrate through a desired produced substance, 2) selecting the same number of independent metabolic fluxes from all metabolic fluxes as the degree of freedom of the stoichiometric matrix as free fluxes, 3) creating a sufficient number of random combinations of the free fluxes for a statistical analysis and calculating a metabolic flux distribution from each created combination based on the stoichiometric matrix, 4) obtaining a regression equation including a minimum number of free fluxes that shows a correlation with substance production from the calculated metabolic flux distributions by a multivariate statistical analysis, and 5) determining at least one metabolic flux affecting substance production based on a coefficient in the obtained regression equation.

Method for determining metabolic flux affecting substance production

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Kawasaki, Japan

Takuji Ueda