Riken Corporation

Taku Demura

Misato Ohtani

Tadao Asami

Hiroyuki Osada

Takeshi Nakano

Keiji Numata

Ayumi Yamagami

Takeshi Yoshizumi

Yutaka Kodama

Takashi Yamamoto

Tetsushi Sakuma

Hiroshi Ezura

Shigeru Sato

Yoko Horii

Kenji Miura

Satoshi Endo

Nobuyuki Nishikubo

This invention relates to an agent for inducing a callus comprising a compound represented by Formula (I) or a hydrolysis product of an amide bond thereof: wherein Arrepresents phenyl substituted with substituent or substituents selected from alkoxy and methylenedioxy; Arrepresents phenyl substituted with halogen; Rand Reach represent hydrogen, alkyl, cyano, or carboxyl; Rand Rmay together form oxo; Rto Reach represent hydrogen or methyl; and Rand R, Rand R, Rand R, and/or Rand Rmay together form oxo; a method for inducing a callus and a method for plant transformation using such agent for inducing a callus.

Agent for inducing callus and method for inducing callus

It is an object of the present invention to provide a method for introducing a protein into a plant, which is simple and extensively applicable to various types of plant cells and proteins. The above object is achieved by the present invention to provide a complex comprising a protein of interest to be introduced into a target plant cell and a carrier peptide, a method for introducing a protein of interest into a target plant cell using the complex, and a kit comprising a protein of interest to be introduced into a target plant cell and a carrier peptide.

Method for introducing protein into plant cell

The object of the present invention is to provide a method of introducing a nucleic acid into plant cells, which is simple and widely applicable to various types of plant cells and nucleic acids. The present invention relates to a method of introducing a nucleic acid into a target plant cell, comprising a step of forming a complex by bringing a carrier peptide, wherein the carrier peptide comprises a cell-penetrating sequence and a polycationic sequence, into contact with a nucleic acid and a step of bringing the obtained complex into contact with a target plant cell.

Method of introducing nucleic acid into plant cells

Provided is a method for increasing the thickness of the secondary cell wall of a plant. A method for increasing the thickness of the secondary cell wall of a plant by using a tracheary element differentiation (TED)-associated protein or a C-terminal fragment of the same, which comprises constructing a transgenic (Tg) plant containing, in an expressible manner, a DNA encoding protein(s) selected from the group consisting of a combination of TED6 with TED7, a C-terminal fragment of TED6, a C-terminal fragment of TED7, and a combination of a C-terminal fragment of TED6 with a C-terminal fragment of TED7, and expressing said DNA in said plant; the Tg plant; and a progeny, a cell, a tissue or a seed of the same.

Method for promoting the formation of secondary cell wall of plant

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Wako, Japan

Taku Demura