Chugai Seiyaku Kabushiki Kaisha

Fuji Photo Film Company, Limited

Pharmalogicals Research Pte. Ltd.

Takeshi Watanabe

Masami Suzuki

Atsuhiko Kato

Tatsumi Yamazaki

Hisafumi Okabe

Koichi Matsubara

Shinta Kobayashi

Osamu Natori

Hiroaki Nakamura

Shiro Kaneko

An objective of the present invention is to obtain two types of substantively homogeneous cancer stem cell populations which can be characterized using the cell surface marker Lgr5, and to provide cancer therapeutics using an antibody against a cell membrane molecule specifically expressed in these cancer stem cells by identifying said cell membrane molecule. A further objective is to provide, using an antibody against a cell membrane molecule specifically expressed in cancer stem cells, a reagent for detecting cancer stem cells, and a method for diagnosing and sorting cancer patients. The present inventors discovered that highly pure large intestine cancer stem cells (CSC) can be obtained in a large quantity, and identified the two types of conditions of large intestine CSCs distinguishable through Lgr5 expression. Moreover, the present inventors discovered that an antibody against a cell membrane molecule specifically expressed in said cancer stem cells can damage said cells.

Cancer stem cell-specific molecule

An objective of the present invention is to provide: a cancer stem cell isolated using a cell marker; a substantively homogeneous cancer stem cell population including said cancer stem cell; and a method of preparing said cancer stem cell population. Another objective of the present invention is to provide: a method for separating cancer stem cells with a high proliferative potential and those with a low proliferative potential; a method for inducing cancer stem cells to have a different proliferative potential; and cancer stem cells separated or induced by these separation or induction methods. A further objective of the present invention is to provide: a method of screening for pharmaceuticals using said cancer stem cell or cancer stem cell population; a method for detecting the presence of said cancer stem cell or cancer stem cell population and for identifying or quantifying the same. The present inventors discovered that highly pure colon cancer stem cells (CSC) can be obtained in a large quantity, and identified two types of states of colon CSCs distinguishable through Lgr5 expression.

Cancer stem cell isolation

A process for producing a pressure-sensitive copying paper which comprises forming a single-layer, free-fall, vertical curtain of a coating solution containing microcapsules as a main component and coating the coating solution onto a continuously running web across the single-layer, free-fall, vertical curtain and a process for producing a pressure-sensitive copying paper which comprises forming a dual-layer, free-fall, vertical curtain of a coating solution containing microcapsules as a main component and a coating solution containing a color developer as a main component and coating the coating solutions onto a continuously running web across the dual-layer, free-fall, vertical curtain.

Process for producing pressure-sensitive copying paper

Growing community of inventors

Shizuoka, Japan

Takeshi Watanabe