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Sapporo Breweries Limited

Takeshi Uozumi

Makoto Hidaka

Norio Shimizu

Teruhiko Beppu

Yasuo Yoneta

Katsuhiko Nishimori

Noboru Yanagida

Haruhiko Masaki

Yoshiyuki Kawaguchi

Akira Nakamura

Michihisa Maeda

An isolated structural Bacillus sp. TB-90 (FERM BP-795) urease gene, which comprises base sequences encoding the amino acid sequences of three subunits of urease. A recombinant DNA comprising Bacillus sp. TB-90 (FERM BP-795) urease gene capable of replicating in Escherichia coli. A process for producing urease, which comprises cultivating Escherichia coli carrying a recombinant DNA comprising Bacillus sp. TB-90 (FERM BP-795) urease gene capable of replicating in Escherichia coli and recovering urease from the culture mixture

Gene and process for producing a thermostable urease

Expression plasmids containing the full cDNA sequence of calf prochymosin and capable of expressing prochymosin gene in E. coli host cells are disclosed. A method is described for the preparation of said plasmids which comprises altering the spacing between the SD and ATG sequences within the E. coli trp promoter-operator region of the parent expression plasmid, pCR 701, which is known to express prochymosin gene in the E. coli host cells. These modified expression plasmids are designed to provide high expression levels of prochymosin.

Novel expression plasmids containing the full cDNA sequence of calf

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Tokyo, Japan

Takeshi Uozumi