Takeda Chemical Industries, Inc.

Takeshi Takahashi

Takashi Suzuki

Koichi Kato

Itsuo Furuoya

Kazunori Hatano

Yoshio Yamazaki

Kazuo Nakahama

Kenji Kawahara

Masaharu Tokuyama

Toshiyuki Takahashi

The present invention relates to a DNA fragment containing a gene encoding acylamino acid racemase. The acylamino acid racemase can be produced industrially at high efficiency using the DNA fragment.

DNA Fragment encoding acylamino acid racemase

A novel choline oxidase being thermostable at 50.degree. C. at pH 7 to 9 is disclosed. A method for producing the enzyme by using actinomycetes is also disclosed.

Choline oxidase and method for producing the same

Novel choline oxidase and method for producing the same

The present invention relates to acylamino acid racemase, production and use thereof. The acylamino acid racemase of the present invention racemizes optically active N-acyl-.alpha.-aminocarboxylic acid alone at pH values around the neutral level at a normal temperature under normal pressure in the presence of optical active amino acid; its use in combination with D- or L-aminoacylase enables the production of optically active D- or L-.alpha.-amino acid from DL-acyl-.alpha.-aminocarboxylic acid at a high level of efficiency.

Acylamino acid racemase, production and use thereof

.beta.-1,3-Glucan derivatives, which are produced by reacting a water-insoluble .beta.-1,3-glucan with a cyanogen halide, are a useful water-insoluble carrier of high reactivity for the production of water-insoluble enzymes and of carrier-ligand products suitable for affinity chromatography.

.beta.-1,3-Glucan derivatives

A new method for production of a cephalosporin ester (I) which comprises reacting a derivative of an organic acid (II) with a 7-aminocephem compound (III) in the presence of an enzyme of a microorganism of one of the genera Escherichia, Bacillus, Proteus and Pseudomonas: ##SPC1## ##EQU1## ##SPC2## wherein R is an unsaturated six-membered carbocyclic ring or five-membered heterocyclic ring residue which may optionally have uncharged substituent groups; R.sup.1 is hydrogen or an uncharged substituent group such as hydroxyl or halogen; R.sup.2 is a hydrogen atom or an organic group which is bound through an oxygen, sulfur or nitrogen atom; COOR.sup.3 is a group which may easily be converted to COOH.

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Izumi, Japan

Takeshi Takahashi