Juridical Foundation the Chemo-sero-therapeutic Research Institute

Other

Takeshi Mikami

Kozo Fujisaki

Ikuo Igarashi

Hideyuki Nagasawa

Hiromi Ikadai

Naoyoshi Suzuki

Hiroaki Maeda

Sachio Tokiyoshi

Kazuhiko Kimachi

Kiyoto Nishiyama

Yukinobu Tohya

The present invention provides a gene encoding a protein from merozoite of, a recombinant protein of, and an antibody capable specifically binding to a 48 kDa protein of rhoptry ofmerozoite. In accordance with the present invention, it is possible to stably prepare the 48 kDa protein of rhoptry ofand the gene encoding said protein in a large amount with the recombinant DNA technique. The present invention also provides a method for diagnosing equine babesiasis which comprises either specifically detecting anti-antibody present in equine blood by using the recombinant protein of present invention as an antigen or detecting the presence ofmerozoite in equine blood by using the antibody of the present invention.

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The present invention provides a gene encoding a protein from merozoite of, a recombinant protein of, and an antibody capable specifically binding to a 48 kDa protein of rhoptry ofmerozoite. In accordance with the present invention, it is possible to stably prepare the 48 kDa protein of rhoptry ofand the gene encoding said protein in a large amount with the recombinant DNA technique. The present invention also provides a method for diagnosing equine babesiasis which comprises either specifically detecting anti-antibody present in equine blood by using the recombinant protein of present invention as an .antigen or detecting the presence ofmerozoite in equine blood by using the antibody of the present invention.

Gene encoding merozoite protein of Babesia caballi, recombinant protein obtained therefrom and utilization thereof

Anti-FCV (feline calicivirus) feline-type recombinant antibody effective for treatment, prevention and diagnosis of FCV infection and a gene fragment useful for preparation of said antibody are provided. Cell line 1D7 capable of producing a mouse monoclonal antibody having an excellent FCV-neutralizing activity was constructed and a gene fragment coding for the V region in charge of the FCV-specific binding of said antibody was obtained. This gene fragment and the gene coding for the constant region of the feline antibody are used to give a chimeric anti-FCV recombinant antibody. The obtained recombinant antibody is a novel antibody and is useful for the diagnosis, treatment and prevention of feline virus infections, particularly feline calicivirus infection, with high safety in administration into cats.

Anti-feline calicivirus recombinant antibody and gene fragment encoding

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Hokkaido, Japan

Takeshi Mikami