Sumitomo Chemical Company, Limited

Takeshi Ishii

Satoshi Mitsuda

Takeshi Sugai

Hiromichi Ohta

Hiroyuki Asako

Yoshikazu Fukita

An isolated esterase gene coding for an esterase capable of causing asymmetric hydrolysis of an organic carboxylic acid ester of a cyclopentenolone of formula I:wherein Ris hydrogen or methyl, and Ris C-Calkyl, C-Calkenyl, C-Calkynyl, C-Chaloalkyl, a C-Caliphatic hydrocarbon moiety which may be optionally protected on the terminal hydroxyl group thereof, or a C-Cfatty acid residue which may be optionally protected on the terminal carboxyl group thereof, to produce the cyclopentenolone of formula I in (R)-form, and hybridizing to the base sequence of SEQ ID NO:1, is useful for the industrially favorable production of optically active cyclopentenolones of formula I which serve as the intermediates of drugs, agricultural chemicals or other active products.

Esterase gene and its use

An isolated esterase gene coding for an esterase capable of causing asymmetric hydrolysis of an organic carboxylic acid ester of a cyclopentenolone of formula I;

Method for producing optically active cyclopropanecarboxylic acid

An industrially advantageous production method of an optically active alcohol useful for cosmetics and medical supplies or production intermediates thereof using an esterase having an ability to selectively hydrolyze an optically active sphingoid compound.

Production of optically active sphingoid compound

There is disclosed a process for producing a nucleoside derivative of the formula [I]: ##STR1## characterized by contacting 2',3',5'-O-triacylribonucleoside derivative of the formula [II]: ##STR2## with an ester hydrolase: (i) capable of regio-selectively deacylating the acyl group at 5'-O-position in the formula [II] above, and

Process for producing nucleoside derivatives

An isolated esterase gene coding for an esterase capable of causing asymmetric hydrolysis of an organic carboxylic acid ester of a cyclopentenolone of formula I: ##STR1## wherein R.sub.1 is hydrogen or methyl, and R.sub.2 is C.sub.1 -C.sub.10 alkyl, C.sub.2 -C.sub.10 alkenyl, C.sub.2 -C.sub.10 alkynyl, C.sub.1 -C.sub.4 haloalkyl, a C.sub.5 -C.sub.9 aliphatic hydrocarbon moiety which may be optionally protected on the terminal hydroxyl group thereof, or a C.sub.5 -C.sub.9 fatty acid residue which may be optionally protected on the terminal carboxyl group thereof, to produce the cyclopentenolone of formula I in (R)-form, and hybridizing to the base sequence of SEQ ID NO:1, is useful for the industrially favorable production of optically active cyclopentenolones of formula I which serve as the intermediates of drugs, agricultural chemicals or other active products.

Growing community of inventors

Takarazuka, Japan

Takeshi Ishii