Terumo Kabushiki Kaisha

Terumo Corporation

Takeshi Igarashi

Atsushi Shimizu

Toshihiko Endo

Tatsuhiko Ikeda

Isei Koshi

A medium for lysine decarboxylation test comprises 2 to 7 g of yeast extract, 1 to 5 g of peptone, 0.5 to 1.5 g of glucose, 0.3 to 1 g of monalkali metal dihydrogen phosphate, 10 to 40 g of L-lysine mineral acid salt, 0.01 to 0.08 of 5'-pyridoxalphosphoric ester, and 0.04 to 0.1 g of a pH indicator, and has a pH value in the range of 5.5 to 6.5 when dissolved in 2 liter of water.

Medium for lysine decarboxylation test

A pad assembly for use in a microorganism culture test is provided which comprises, in sterile conditions, a water-absorbing pad which is impregnated with a culture medium for culturing given microorganisms and dried, a filter member bonded to the upper surface of said water absorbing pad and having submicron pores of a size capable of substantially preventing the microorganisms from passing therethrough, and a coating of an antibiotic deactivating agent applied to the upper surface of said filter member or to the common surface between said filter member and said water-absorbing pad, as well as testing apparatus having the pad assembly mounted in a vessel. The invention eliminates the adverse effect of antibiotics in a sample to be tested which will otherwise inhibit the growth of microorganisms in the sample to adversely alter test results.

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Tama, Japan

Takeshi Igarashi