Honda Motor Co., Ltd.

Takeshi Ara

Daisuke Shibata

Shigenobu Mitsuzawa

Migiwa Takeda

Satoru Shinkawa

Maiko Tanaka

Maiko Fukuura

A saccharifying enzyme composition, by which excellent saccharification performance can be attained even with a low usage thereof, as well as a method for producing a saccharified solution using the same are provided. The saccharifying enzyme composition subjects lignocellulose-based biomass as a substrate to a saccharification treatment. The saccharifying enzyme composition comprises an endoglucanase not containing a cellulose-binding domain, a cellobiohydrolase containing a cellulose-binding domain, and a &#x3b2;-glucosidase containing a cellulose-binding domain.

Saccharifying enzyme composition and method for producing saccharified solution using the same

The present invention relates to a polypeptide which has &#x3b2;-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 91% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel &#x3b2;-glucosidase enzyme derived from, a polynucleotide encoding the &#x3b2;-glucosidase, an expression vector for expressing the &#x3b2;-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the &#x3b2;-glucosidase can be provided.

&#x3b2;-glucosidase

The present invention relates to a polypeptide which has &#x3b2;-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 90% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel &#x3b2;-glucosidase enzyme derived fromcellulolyticus, a polynucleotide encoding the &#x3b2;-glucosidase, an expression vector for expressing the &#x3b2;-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the &#x3b2;-glucosidase can be provided.

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Kisarazu, Japan

Takeshi Ara