Terumo Corporation

Terumo Kabushiki Kaisha

Meiji Seika Kaisha, Ltd.

Takeo Nomura

Masaki Shimizu

Tadashi Sudo

Chuhei Nojiri

Osamu Makabe

Hiroyuki Hamaguchi

Kozo Nagaoka

Syuji Ichikawa

Shinichiro Kuriya

Keita Matsunaga

Kiyoyuki Ogata

Megakaryocyte colony stimulating factor and process for its preparation

Alpha-hydroperoxyisopropylphenyl compounds and process for preparing the

Hot-water extracts of the neem bark produced by treating the bark of neem with water at a temperature from 0.degree. to 40.degree. C. and subjecting the residue from said pre-extraction treatment to extraction treatment with hot water. Purification of the extract thus produced by means of alcohol precipitation or dialysis gives products of a higher purity. Moreover, treatments prior to the pre-extraction treatment with water at 0.degree.-40.degree. C. with a non-polar organic solvent having a dielectric constant of 10 or lower and a polar organic solvent having a dielectric constant from 15 to 35 yields neem extracts of a further higher purity. The hot-water extracts of the neem bark according to the present invention possess activity against mouse L-5178Y cells and transplanted sarcoma 180 tumors.

Hot-water extracts of neem bark

A hot-water extract of the bark of Melia azadirachta L. is treated with a gel filtrating agent with a fractionation range molecular weight from 1.times.10.sup.3 -1.times.10.sup.5 to 1.times.10.sup.3 -1.5.times.10.sup.5. There is obtained polysaccharide N9GI from the first fraction of the three divided polysaccharide fractions. Polysaccharide N9GI further treated with a gel-filtrating agent with a fractionation range molecular weight from 1.times.10.sup.3 -2.times.10.sup.5 to 1.times.10.sup.3 -8.times.10.sup.5. There are obtained polysaccharide N9GIa and polysaccharide N9GIb respectively from the first and second fractions of the two divided polysaccharide fractions.

Polysaccharides N9GI, their preparation and therapeutic compositions

Neem bark extracts produced by pre-treating the bark of neem with an organic solvent having a dielectric constant of 10 or lower, for example, benzene or ethyl acetate and subjecting the residue obtained by the pre-treatment to extraction with a hydrophilic organic solvent having a dielectric constant from 15 to 35, for example, methanol or ethanol and recovering the neem bark extracts from the hydrophilic organic extraction solvent.

Neem bark extracts

A device for culturing microorganisms includes a vessel having a mouth for containing a culture medium and a stopper member for hermetically sealing the vessel. The stopper member is provided with a through hole on its peripheral wall for communicating with the inner atmosphere of said vessel.

Microorganism culturing device

Growing community of inventors

Hino, Japan

Takeo Nomura