Other

Yokoyama, Shigeyuki

Takehiro Shinoda

Shigeyuki Yokoyama

Mikako Shirouzu

Kaori Ito

Yoshihiro Nakamura

Naoko Nomura

Hiroaki Tanabe

Tetsuya Hori

Yoshiko Katsura

Tomomi Someya

In order to provide a membrane protein production method which does not require the step of solubilizing a membrane protein and which allows the membrane protein having an excellent quality to be obtained with a high yield, a method in accordance with an embodiment of the present invention includes: a step (a) of preparing a reaction solution for cell-free protein synthesis, the reaction solution containing (i) a template nucleic acid which encodes the membrane protein, (ii) a lipid, and (iii) a detergent which is contained at a concentration equal to or higher than a critical micelle concentration; and a step (b) of synthesizing the membrane protein while the concentration of the detergent in the reaction solution is maintained at a concentration equal to or higher than a critical micelle concentration.

Method of manufacturing membrane protein and utilization thereof

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Saitama, Japan

Takehiro Shinoda