Canon U.S. Life Sciences, Inc.

Takayoshi Hanagata

Kenton C Hasson

Ivor T Knight

Weidong Cao

Hiroshi Inoue

Brian P Murphy

Eric D Schneider

Johnathan Stuart Coursey

Shulin Zeng

Alex K Flamm

Ben Lane

Gregory H Owen

Scott Corey

Sami Kanderian

Conrad Laskowski

Hongye Liang

Brian Bean

Franklin Regan

Ying-Xin Wang

Jeffrey Frederick Miller

Eric Chan

Gregory A Dale

Chris Felice

Ronald Kurz

Kazuhiko Hasegawa

Ralph McCann

Jason Clemons

Shigeru Gary Natsume

Justin Waldinger

The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.

System and method for serial processing of multiple nucleic acid assays

The invention relates to methods and devices for control of an integrated thin-film device with a plurality of microfluidic channels. In one embodiment, the microfluidic device includes a microfluidic chip comprising a first zone having a plurality of microfluidic channels and a second zone having a plurality of microfluidic channels, wherein the microfluidic channels in the first and second zones are in fluid communication. The microfluidic device further comprising a thin-film heater in thermal communication with each of the microfluidic channels in the first and second zones. The microfluidic device also includes a control system configured to independently control the temperature of each of the thin-film heaters using pulse width modulation (PWM) control signals that are optimized for each of the thin-film heaters.

Microfluidic devices with integrated resistive heater electrodes including systems and methods for controlling and measuring the temperatures of such heater electrodes

Cartridge assembly

Analysis instrument

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Newport News, VA, United States of America

Takayoshi Hanagata