Eisai Company, Limited

Eisai R&d Management Co., Ltd.

Eisai Research Institute

Takashi Yoshinaga

Kohei Sawada

Fumihiro Ozaki

Kiyoaki Kobayashi

Tatsuhiro Onogi

Hiroyuki Suzuki

Koki Kawano

Yoshihiko Norimine

Mutsuko Ono

Hiroe Minami

Toshihiko Kaneko

Yoshinori Takahashi

Masayuki Matsukura

Kazuki Miyazaki

Ichiro Yoshida

Hiroki Ishihara

Junichi Kamata

Norimasa Miyamoto

Mitsuhiro Ino

Isao Tanaka

Hiroshi Katoh

Mutsuko Tabata

Toru Arai

Eiki Takahashi

Toru Oki

Shinji Hatakeyama

Tetsuhiro Niidome

Yukio Nishizawa

Miyuki Ohkubo

It is an object of the present invention to establish a method of establishing a cell with a remarkably high hERG channel expression level for use in predicting adverse effects based on hERG channel inhibition in research and development of drugs, and to thereby establish a highly sensitive and high throughput evaluation method. By inserting a hERG gene into a retrovirus vector plasmid or lentivirus vector plasmid to thereby prepare a virus vector, concentrating the vector by ultracentrifugation if necessary, transferring the hERG gene into cells and expressing hERG channels therein, it has become possible to secure an expression level effective in measurements using a fully automated high throughput patch clamp system or dyes.

hERG channel-expressing cell

Compounds represented by the following general formula: [wherein X, X, Xand Xeach independently represent a single bond, Calkylene, etc.; Arepresents optionally substituted phenyl, etc.; Arepresents an optionally substituted 5- to 7-membered heterocyclic group containing &#x2014;C(=Q)- (wherein Qrepresents oxygen, sulfur or &#x2550;N&#x2014;R(wherein Rrepresents hydrogen or Calkyl)) and nitrogen, etc.; and Zrepresents piperidin-diyl, etc.],salts thereof and hydrates of the foregoing.

Nitrogen containing heterocyclic compounds and medicines containing the same

A non-human animal in which a gene coding for the N-type calcium channel is disrupted to lack functional N-type calcium channel, and a method for screening for a substance having a pharmacological action on blood pressure control, transmission of pain, blood sugar level control and so forth by using the animal.

N-calcium channel knockout animal

The present invention provides compounds represented by the following formula:wherein X, X, Xand Xeach independently represents a single bond, Calkylene, etc.; Arepresents optionally substituted phenyl, etc.; Arepresents an optionally substituted 5- to 7-membered heterocyclic group containing &#x2014;C(&#x2550;Q)&#x2014;, Qwherein represents oxygen, sulfur or &#x2550;N&#x2014;R, wherein Rrepresents hydrogen or Calkyl etc.; and Zrepresents piperidin-diyl, ect, salts thereof and hydrates of the foregoing.

Piperidine compound and pharmaceutical composition thereof

Growing community of inventors

Tsukuba, Japan

Takashi Yoshinaga