Takara Bio Inc.

Takara Shuzo Co., Inc.

Kyushu University

Other

The Johns Hopkins University

Rutgers, the State University of New Jersey

Educational Corporation Kansai Bunri Sougougakuen

Takashi Uemori

Ikunoshin Kato

Hiroyuki Mukai

Kiyozo Asada

Yoshimi Sato

Nobuto Koyama

Yoshizumi Ishino

Kazue Miyake

Nariaki Takatsu

Kiyoyuki Matsumura

Takashi Ueno

Tomoko Fujita

Shigekazu Hokazono

Kimikazu Hashino

Osamu Takeda

Kazue Nishiwaki

Eiji Kobayashi

Junko Yamamoto

Takeshi Yamagami

Miwa Akitomo

Nana Shimada

Hiroaki Sagawa

Hikaru Takakura

Michio Hagiya

Hiroshi Kobori

Tetsuki Tanaka

Sonoko Ishino

Keiko Kubo

Ryo Hirano

Yuji Hashimoto

Kayo Fujita

Hiroyuki Matsumoto

Kensuke Saito

Takehiro Sagara

Masayori Inouye

Tatsuji Enoki

Jun Tomono

Fusao Kimizuka

Masahiro Endo

Christian B Anfinsen

Sangita Phadtare

Hiroaki Matsukawa

Kenneth Laderman

Tsuyoshi Shirai

Kikyozo Asada

Yuki Ueda

Kanako Usui

Ling Zhu

Mariko Moriyama

Satoshi Yanagi

Haruhisa Sawaragi

Kazuhiko Ishikawa

Yuko Nakabayashi

Mariko Okawa

Kimakazu Hasino

Provided are: a DNA polymerase mutant having reverse transcriptase activity, the DNA polymerase mutant including a sequence consisting of twelve specific amino acids A1-A12, wherein the DNA polymerase mutant having reverse transcriptase activity is characterized in that the A3 and/or A10 amino acid is substituted by a basic amino acid residue different from that prior to the introduction of mutation; a kit and a composition including the DNA polymerase; a method for producing the DNA polymerase; and a method for modifying an existing DNA polymerase having reverse transcriptase activity.

DNA polymerase mutant suited to nucleic acid amplification from RNA

The present invention pertains to: an oligonucleotide preservation method; and a kit comprising an oligonucleotide. The present invention provides a method for stably preserving an oligonucleotide-containing solution by adding a nucleic acid-binding protein to said oligonucleotide-containing solution in advance.

Oligonucleotide preservation method

Provided are: a reverse transcriptase mutant including an amino acid mutation at a position corresponding to position 55 of the amino acid sequence of wild-type reverse transcriptase derived from the Moloney murine leukemia virus, wherein the reverse transcriptase mutant is characterized in that the amino acid mutation is a substitution from threonine to another amino acid, and the other amino acid is selected from the group consisting of amino acids having a nonpolar aliphatic side chain and amino acids having a polar acidic functional group side chain; a nucleic acid that encodes the mutant; a method for producing the mutant and the nucleic acid that encodes the mutant; a method for synthesizing cDNA in which the mutant is used; and a composition and kit including the mutant.

Heat-resistant reverse transcriptase mutant

The present invention relates to a(Taq) polymerase having a strand displacement activity in which an amino acid residue in a template DNA binding site of the DNA polymerase is substituted with an amino acid to increase a total charge in the site, a nucleic acid encoding the polymerase, a vector containing the nucleic acid, a transformant containing the vector containing the nucleic acid or the nucleic acid, a method for producing the polymerase, a method for amplifying nucleic acids utilizing the polymerase, and a kit containing the polymerase. According to the present invention, a DNA polymerase having a high thermostability, capable of efficiently replicating a long-strand of a template DNA, and having a strong strand displacement activity is provided.

DNA polymerase variant

A polypeptide having a mismatch endonuclease activity of recognizing a mismatch and cleaving the mismatch; a mismatch-specific cleavage reaction using the polypeptide; a method for removing an error in a nucleic acid amplification reaction utilizing the polypeptide; a method for inhibiting the amplification of a nucleic acid comprising a specific nucleotide sequence during a nucleic acid amplification reaction; and a method for detecting a nucleic acid having a single-nucleotide polymorphism mutation utilizing the inhibition method.

Methods of utilizing thermostable mismatch endonuclease

A composition for use in amplifying cDNA synthesized by a reverse transcription reaction and detecting RNA that serves as a template of the reverse transcription reaction, the composition containing a thermostable DNA polymerase, a thermostable ribonuclease H, and an intercalating dye. Since the composition of the present invention can suppress the influences to the nucleic acid amplification reaction by RNA that serves as a template for cDNA synthesis, the composition is useful in the detection of RNA, and more useful in quantification of RNA having a desired sequence by real-time RT-PCR.

Composition for detection of RNA

Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.

Method for using heat-resistant mismatch endonuclease

Provided are the following: a method, for improving reactivity of an acid synthesis reaction, comprising a step for adding an &#x3c9;-amino acid to a reaction solution; a composition, for a nucleic acid synthesis reaction, comprising DNA polymerase, reaction buffer, at least one primer, at least one deoxyribonucleoside triphosphate, and an &#x3c9;-amino acid; and a reaction buffer, for a nucleic acid synthesis reaction, comprising an &#x3c9;-amino acid.

Method for improving nucleic acid synthesis reaction

Provided are the following: a method, for improving reactivity of a nucleic acid synthesis reaction, comprising a step for adding an &#x3c9;-amino acid to a reaction solution; a composition, for a nucleic acid synthesis reaction, comprising DNA polymerase, reaction buffer, at least one primer, at least one deoxyribonucleotide triphosphate, and an &#x3c9;-amino acid; and a reaction buffer, for a nucleic acid synthesis reaction, comprising an &#x3c9;-amino acid.

The present invention pertains to a method for modifying a nucleic acid contained in a sample comprising a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, comprising the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods.

Method for modifying nucleic acids

The present invention pertains to: a method for modifying a nucleic acid contained in a sample, the method including a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, the method including the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods.

Growing community of inventors

Otsu, Japan

Takashi Uemori