Jtec Corporation

Osaka University

Takashi Tsumura

Toshimasa Uemura

Hiromi Okada

Yoshimi Oyabu

Kazuto Yamauchi

Takahiro Ono

Yui Onomura

Takashi Kimura

Provided is a method for efficiently culturing pluripotent stem cells with higher safety. The present invention relates to a method for culturing pluripotent stem cells, the method comprising culturing an isolated pluripotent stem cells in a pseudo-microgravity environment to proliferate the pluripotent stem cells while maintaining the pluripotent stem cells in an undifferentiated state, thereby forming and growing spheroids of the pluripotent stem cells; and a method for inducing differentiation of pluripotent stem cells by using the method.

Method for culturing pluripotent stem cells

Disclosed is a rotating culture vessel based on a rotating culture technology using an RWV, by which cell seeding, liquid medium exchange, quality control and so on can be automated and degassing can be conducted simultaneously with liquid medium exchange without disturbing the cells under culture. Also disclosed is an automatic cell culture apparatus using the same. A rotating culture vessel, which contains cells and a liquid culture medium, to be attached to a horizontal rotating shaft of a rotating culture device to three-dimensionally culture the cells, wherein one or more inlets/outlets for supplying cells and a liquid culture medium at the early stage and then taking out the cultured cells, are formed at appropriate position of a flat cylindrical culture container; at least one pair of a supply port and a discharge port for liquid medium exchange is provided on the outer circumferential cylindrical face of the culture container.

Rotating culture vessel and automatic cell culture apparatus using same

The device is configured from: a reflective surface shape controllable mirror in which a band-shaped X-ray reflective surfaceis formed on a central portion of a front surface of a substrate, reference planesare formed along both sides of the X-ray reflective surface, and a plurality of piezoelectric elementsare attached to at least one of front and back surfaces of the substrate so as to be arranged in the longitudinal direction of the X-ray reflective surface on both side portions of the substrate, and a multichannel control system for applying a voltage to each of the piezoelectric elements.

Reflective surface shape controllable mirror device, and method for manufacturing reflective surface shape controllable mirror

Provided is a small-scale cell culture apparatus having a culture medium replacement function for culturing cells in a small number of Petri dishes, the cell culture apparatus being capable of performing automatic replacement of the culture medium and cell culture within the same cavity and significantly reducing the apparatus cost. At least one set of upper and lower discs is arranged inside an incubator chamber. Petri dishesin each of which cells and a culture medium are put are held on lower discs, and top lidsare held on upper discs. The lower side of each of the top lids is open.

Growing community of inventors

Kobe, Japan

Takashi Tsumura